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Title: Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: Model systems for equine sarcoids

Abstract

It is now widely recognized that BPV-1 and less commonly BPV-2 are the causative agents of equine sarcoids. Here we present the generation of equine cell lines harboring BPV-1 genomes and expressing viral genes. These lines have been either explanted from sarcoid biopsies or generated in vitro by transfection of primary fibroblasts with BPV-1 DNA. Previously detected BPV-1 genome variations in equine sarcoids are also found in sarcoid cell lines, and only variant BPV-1 genomes can transform equine cells. These equine cell lines are morphologically transformed, proliferate faster than parental cells, have an extended life span and can grow independently of substrate. These characteristics are more marked the higher the level of viral E5, E6 and E7 gene expression. These findings confirm that the virus has an active role in the induction of sarcoids and the lines will be invaluable for further studies on the role of BPV-1 in sarcoid pathology.

Authors:
; ; ; ;  [1]
  1. Institute of Comparative Medicine, University of Glasgow Faculty of Veterinary Medicine, Bearsden Road, Glasgow G61 1QH (United Kingdom)
Publication Date:
OSTI Identifier:
21140969
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 373; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2007.11.037; PII: S0042-6822(07)00806-9; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOPSY; CATTLE; DNA; FIBROBLASTS; GENES; IN VITRO; IN VIVO; LIFE SPAN; PATHOLOGY; SUBSTRATES; VIRUSES

Citation Formats

Yuan, Z Q, Gault, E A, Gobeil, P, Nixon, C, Campo, M S, and Nasir, L. Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: Model systems for equine sarcoids. United States: N. p., 2008. Web. doi:10.1016/j.virol.2007.11.037.
Yuan, Z Q, Gault, E A, Gobeil, P, Nixon, C, Campo, M S, & Nasir, L. Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: Model systems for equine sarcoids. United States. https://doi.org/10.1016/j.virol.2007.11.037
Yuan, Z Q, Gault, E A, Gobeil, P, Nixon, C, Campo, M S, and Nasir, L. 2008. "Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: Model systems for equine sarcoids". United States. https://doi.org/10.1016/j.virol.2007.11.037.
@article{osti_21140969,
title = {Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: Model systems for equine sarcoids},
author = {Yuan, Z Q and Gault, E A and Gobeil, P and Nixon, C and Campo, M S and Nasir, L.},
abstractNote = {It is now widely recognized that BPV-1 and less commonly BPV-2 are the causative agents of equine sarcoids. Here we present the generation of equine cell lines harboring BPV-1 genomes and expressing viral genes. These lines have been either explanted from sarcoid biopsies or generated in vitro by transfection of primary fibroblasts with BPV-1 DNA. Previously detected BPV-1 genome variations in equine sarcoids are also found in sarcoid cell lines, and only variant BPV-1 genomes can transform equine cells. These equine cell lines are morphologically transformed, proliferate faster than parental cells, have an extended life span and can grow independently of substrate. These characteristics are more marked the higher the level of viral E5, E6 and E7 gene expression. These findings confirm that the virus has an active role in the induction of sarcoids and the lines will be invaluable for further studies on the role of BPV-1 in sarcoid pathology.},
doi = {10.1016/j.virol.2007.11.037},
url = {https://www.osti.gov/biblio/21140969}, journal = {Virology},
issn = {0042-6822},
number = 2,
volume = 373,
place = {United States},
year = {Thu Apr 10 00:00:00 EDT 2008},
month = {Thu Apr 10 00:00:00 EDT 2008}
}