Recombinant Human Epidermal Growth Factor Accelerates Recovery of Mouse Small Intestinal Mucosa After Radiation Damage

Lee, Kang Kyoo; Jo, Hyang Jeong; Hong, Joon Pio; Lee, Sang-wook; Sohn, Jung Sook; Moon, Soo Young; Yang, Sei Hoon; Shim, Hyeok; Lee, Sang Ho; Ryu, Seung-Hee; Moon, Sun Rock

doi:10.1016/j.ijrobp.2008.03.041

Title: Recombinant Human Epidermal Growth Factor Accelerates Recovery of Mouse Small Intestinal Mucosa After Radiation Damage

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Abstract

Purpose: To determine whether systemically administered recombinant human epidermal growth factor (rhEGF) accelerates the recovery of mouse small intestinal mucosa after irradiation. Methods and Materials: A mouse mucosal damage model was established by administering radiation to male BALB/c mice with a single dose of 15 Gy applied to the abdomen. After irradiation, rhEGF was administered subcutaneously at various doses (0.04, 0.2, 1.0, and 5.0 mg/kg/day) eight times at 2- to 3-day intervals. The evaluation methods included histologic changes of small intestinal mucosa, change in body weight, frequency of diarrhea, and survival rate. Results: The recovery of small intestinal mucosa after irradiation was significantly improved in the mice treated with a high dose of rhEGF. In the mice that underwent irradiation without rhEGF treatment, intestinal mucosal ulceration, mucosal layer damage, and severe inflammation occurred. The regeneration of villi was noticeable in mice treated with more than 0.2 mg/kg rhEGF, and the villi recovered fully in mice given more than 1 mg/kg rhEGF. The frequency of diarrhea persisting for more than 3 days was significantly greater in the radiation control group than in the rhEGF-treated groups. Conclusions: Systemic administration of rhEGF accelerates recovery from mucosal damage induced by irradiation. We suggest thatmore »« less

Authors:

Lee, Kang Kyoo ^[1]; Jo, Hyang Jeong ^[2]; Hong, Joon Pio ^[3]; Lee, Sang-wook ^[4]; Sohn, Jung Sook ^[5]; Moon, Soo Young ^[4]; Yang, Sei Hoon; Shim, Hyeok ^[6]; Lee, Sang Ho ^[7]; Ryu, Seung-Hee ^[4]; Moon, Sun Rock ^[1]

Department of Radiation Oncology, University of Wonkwang School of Medicine, Iksan (Korea, Republic of)
Department of Pathology, University of Wonkwang School of Medicine, Iksan (Korea, Republic of)
Department of Plastic and Reconstructive Surgery, University of Ulsan College of Medicine, Asan Medical Center, Seoul (Korea, Republic of)
Department of Radiation Oncology, University of Ulsan College of Medicine, Asan Medical Center, Seoul (Korea, Republic of)
Vestibulocochlear Research Center, University of Wonkwang School of Medicine, Iksan (Korea, Republic of)
Department of Internal Medicine, University of Wonkwang School of Medicine, Iksan (Korea, Republic of)
Department of Radiology, Iksan General Hospital, Iksan (Korea, Republic of)

Publication Date:: Tue Jul 15 00:00:00 EDT 2008

OSTI Identifier:: 21124373

Resource Type:: Journal Article

Journal Name:: International Journal of Radiation Oncology, Biology and Physics

Additional Journal Information:: Journal Volume: 71; Journal Issue: 4; Other Information: DOI: 10.1016/j.ijrobp.2008.03.041; PII: S0360-3016(08)00537-3; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0360-3016

Country of Publication:: United States

Language:: English

Subject:: 62 RADIOLOGY AND NUCLEAR MEDICINE; ABDOMEN; BIOLOGICAL RADIATION EFFECTS; DIARRHEA; ENTERITIS; GROWTH FACTORS; INFLAMMATION; IRRADIATION; MICE; MUCOUS MEMBRANES; RADIATION DOSES

Citation Formats


                    Lee, Kang Kyoo, Jo, Hyang Jeong, Hong, Joon Pio, Lee, Sang-wook, Sohn, Jung Sook, Moon, Soo Young, Yang, Sei Hoon, Shim, Hyeok, Lee, Sang Ho, Ryu, Seung-Hee, and Moon, Sun Rock. Recombinant Human Epidermal Growth Factor Accelerates Recovery of Mouse Small Intestinal Mucosa After Radiation Damage.  United States: N. p., 2008. 
        Web.  doi:10.1016/j.ijrobp.2008.03.041.

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                    Lee, Kang Kyoo, Jo, Hyang Jeong, Hong, Joon Pio, Lee, Sang-wook, Sohn, Jung Sook, Moon, Soo Young, Yang, Sei Hoon, Shim, Hyeok, Lee, Sang Ho, Ryu, Seung-Hee, & Moon, Sun Rock. Recombinant Human Epidermal Growth Factor Accelerates Recovery of Mouse Small Intestinal Mucosa After Radiation Damage.  United States.  https://doi.org/10.1016/j.ijrobp.2008.03.041

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                    Lee, Kang Kyoo, Jo, Hyang Jeong, Hong, Joon Pio, Lee, Sang-wook, Sohn, Jung Sook, Moon, Soo Young, Yang, Sei Hoon, Shim, Hyeok, Lee, Sang Ho, Ryu, Seung-Hee, and Moon, Sun Rock. 2008.  
        "Recombinant Human Epidermal Growth Factor Accelerates Recovery of Mouse Small Intestinal Mucosa After Radiation Damage".  United States.  https://doi.org/10.1016/j.ijrobp.2008.03.041.

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@article{osti_21124373,

  title        = {Recombinant Human Epidermal Growth Factor Accelerates Recovery of Mouse Small Intestinal Mucosa After Radiation Damage},

  author       = {Lee, Kang Kyoo and Jo, Hyang Jeong and Hong, Joon Pio and Lee, Sang-wook and Sohn, Jung Sook and Moon, Soo Young and Yang, Sei Hoon and Shim, Hyeok and Lee, Sang Ho and Ryu, Seung-Hee and Moon, Sun Rock},

  abstractNote = {Purpose: To determine whether systemically administered recombinant human epidermal growth factor (rhEGF) accelerates the recovery of mouse small intestinal mucosa after irradiation. Methods and Materials: A mouse mucosal damage model was established by administering radiation to male BALB/c mice with a single dose of 15 Gy applied to the abdomen. After irradiation, rhEGF was administered subcutaneously at various doses (0.04, 0.2, 1.0, and 5.0 mg/kg/day) eight times at 2- to 3-day intervals. The evaluation methods included histologic changes of small intestinal mucosa, change in body weight, frequency of diarrhea, and survival rate. Results: The recovery of small intestinal mucosa after irradiation was significantly improved in the mice treated with a high dose of rhEGF. In the mice that underwent irradiation without rhEGF treatment, intestinal mucosal ulceration, mucosal layer damage, and severe inflammation occurred. The regeneration of villi was noticeable in mice treated with more than 0.2 mg/kg rhEGF, and the villi recovered fully in mice given more than 1 mg/kg rhEGF. The frequency of diarrhea persisting for more than 3 days was significantly greater in the radiation control group than in the rhEGF-treated groups. Conclusions: Systemic administration of rhEGF accelerates recovery from mucosal damage induced by irradiation. We suggest that rhEGF treatment shows promise for the reduction of small intestinal damage after irradiation.},

  doi          = {10.1016/j.ijrobp.2008.03.041},

  url          = {https://www.osti.gov/biblio/21124373},
  journal      = {International Journal of Radiation Oncology, Biology and Physics},

  issn         = {0360-3016},

  number       = 4,

  volume       = 71,

  place        = {United States},

  year         = {Tue Jul 15 00:00:00 EDT 2008},

  month        = {Tue Jul 15 00:00:00 EDT 2008}

}

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