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Title: Characterisation of electron currents generated by the human neutrophil NADPH oxidase

Electron transport by the human neutrophil NADPH oxidase is an important microbicidal weapon for phagocytes. The electron current (I{sub e}) generated by the neutrophil NADPH oxidase is poorly characterised due to the lack of appropriate electrophysiological data. In this study, I fully characterise the neutrophil generated I{sub e} when the NADPH oxidase is activated by NADPH and GTP{gamma}S. The neutrophil I{sub e} was markedly voltage-dependent in the entire voltage range in comparison to those electron currents measured after chloride was removed from the external bath solution. The difference in I{sub e} measured in chloride free conditions was not due to a change in the activation kinetics of voltage-gated proton channels. The I{sub e} depolarises the neutrophil plasma membrane at a rate of 2.3 V s{sup -1} and this depolarisation was opposed when voltage-gated proton channels are activated. 3 mM ZnCl{sub 2} depolarised the membrane potential to +97.8 {+-} 2.5 mV (n = 4), and this depolarisation was abolished after NADPH oxidase inhibition.
Authors:
 [1]
  1. Leukocyte and Ion Channel Research Laboratory, School of Health and Biosciences, University of East London, Stratford Campus, London E15 4LZ (United Kingdom), E-mail: j.ahluwalia@uel.ac.uk
Publication Date:
OSTI Identifier:
21043702
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 368; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2008.01.161; PII: S0006-291X(08)00190-3; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL MEMBRANES; ELECTRIC POTENTIAL; ELECTRONS; NEUTROPHILS; OXIDASES; PHAGOCYTES; PROTONS; ZINC CHLORIDES