miR-125b inhibits osteoblastic differentiation by down-regulation of cell proliferation

Journal Article · Fri Apr 04 00:00:00 EDT 2008 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2008.01.073· OSTI ID:21043681

Mizuno, Yosuke; Yagi, Ken; Tokuzawa, Yoshimi; Kanesaki-Yatsuka, Yukiko; Suda, Tatsuo ^[1]; Katagiri, Takenobu; Fukuda, Toru ^[2]; Maruyama, Masayoshi; Okuda, Akihiko ^[3]; Amemiya, Tomoyuki; Kondoh, Yasumitsu; Tashiro, Hideo ^[4]; Okazaki, Yasushi ^[1]

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1. Division of Functional Genomics and Systems Medicine, Research Center for Genomic Medicine, Saitama Medical University, 1397-1 Yamane, Hidaka City, Saitama 350-1241 (Japan)
2. Division of Pathophysiology, Research Center for Genomic Medicine, Saitama Medical University, 1397-1 Yamane, Hidaka City, Saitama 350-1241 (Japan)
3. Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical University, 1397-1 Yamane, Hidaka City, Saitama 350-1241 (Japan)
4. Probing Technology Laboratory, RIKEN, 2-1 Hirosawa, Wako City, Saitama 351-0198 (Japan)

Although various microRNAs regulate cell differentiation and proliferation, no miRNA has been reported so far to play an important role in the regulation of osteoblast differentiation. Here we describe the role of miR-125b in osteoblastic differentiation in mouse mesenchymal stem cells, ST2, by regulating cell proliferation. The expression of miR-125b was time-dependently increased in ST2 cells, and the increase in miR-125b expression was attenuated in osteoblastic-differentiated ST2 cells induced by BMP-4. The transfection of exogenous miR-125b inhibited proliferation of ST2 cells and caused inhibition of osteoblastic differentiation. In contrast, when the endogenous miR-125b was blocked by transfection of its antisense RNA molecule, alkaline phosphatase activity after BMP-4 treatment was elevated. These results strongly suggest that miR-125b is involved in osteoblastic differentiation through the regulation of cell proliferation.

• OSTI ID: 21043681
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 368, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2008.01.073; PII: S0006-291X(08)00095-8; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ALKALINE PHOSPHATASE
CELL DIFFERENTIATION
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
GENE REGULATION
MICE
RNA
STEM CELLS
TIME DEPENDENCE