RNA interference targeting tNOX attenuates cell migration via a mechanism that involves membrane association of Rac
- Graduate Institute of Biomedical Sciences, National Chung Hsing University, Taichung 40227, Taiwan (China)
- School of Dentistry, Chung-Shan Medical University, Taichung 402, Taiwan (China)
- Chang-Hwa Hospital, Department of Health, Chang-Hwa 51341, Taiwan (China)
tNOX, a tumor-associated NADH oxidase, is a growth-related protein present in transformed cells. In this study, we employed RNA interference (RNAi)-mediated down-regulation of tNOX protein expression to explore the role of tNOX in regulating cell growth in human cervical adenocarcinoma (HeLa) cells. In this first reported use of RNAi to decrease tNOX expression, we found that HeLa cell growth was significantly inhibited by shRNA-knockdown of tNOX. Furthermore, cell migration and membrane association of Rac were decreased concomitantly with the reduction in tNOX protein expression. These results indicate that shRNA targeting of tNOX inhibits the growth of cervical cancer cells, and reduces cell migration via a decrease in the membrane association of Rac. We propose that tNOX is a potential upstream mediator of Rho activation that plays a role in regulating cell proliferation, migration, and invasion.
- OSTI ID:
- 21043584
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 365, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2007.11.025; PII: S0006-291X(07)02415-1; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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