Analysis of the material properties of early chondrogenic differentiated adipose-derived stromal cells (ASC) using an in vitro three-dimensional micromass culture system
- Pediatric Surgical Research Program and Department of Surgery, Stanford University School of Medicine, 257 Campus Drive, Stanford, CA 94305 (United States)
- Department of Materials Science Engineering, University of California, Berkeley (United States)
Cartilage is an avascular tissue with only a limited potential to heal and chondrocytes in vitro have poor proliferative capacity. Recently, adipose-derived stromal cells (ASC) have demonstrated a great potential for application to tissue engineering due to their ability to differentiate into cartilage, bone, and fat. In this study, we have utilized a high density three-dimensional (3D) micromass model system of early chondrogenesis with ASC. The material properties of these micromasses showed a significant increase in dynamic and static elastic modulus during the early chondrogenic differentiation process. These data suggest that the 3D micromass culture system represents an in vitro model of early chondrogenesis with dynamic cell signaling interactions associated with the mechanical properties of chondrocyte differentiation.
- OSTI ID:
- 20991455
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 359, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2007.05.098; PII: S0006-291X(07)01051-0; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Mitogenic and chondrogenic effects of fibroblast growth factor-2 in adipose-derived mesenchymal cells
Human adipose-derived mesenchymal stromal cells from face and abdomen undergo replicative senescence and loss of genetic integrity after long-term culture