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Title: Nobiletin enhances differentiation and lipolysis of 3T3-L1 adipocytes

Nobiletin is a polymethoxylated flavone found in certain citrus fruits. Here we demonstrate that nobiletin enhance differentiation of 3T3-L1 preadipocytes. Nobiletin dose-dependently increased accumulation of lipid droplets in adipocytes. Quantitative RT-PCR analyses indicated that nobiletin increased the expression of genes critical for acquisition of the adipocyte phenotype. Some of them were known peroxisome proliferator activated receptor {gamma} (PPAR{gamma}) targets and PPAR{gamma} itself, however, nobiletin did not exhibit PPAR{gamma} ligand activity. We observed the expression of CCAAT/enhancer binding protein {beta} (C/EBP{beta}), a transcription factor for PPAR{gamma}, was increased by nobiletin. The activation of cAMP-responsive element binding protein (CREB) and extracellular signal-regulated kinase (ERK), which play important roles in C/EBP{beta} expression were also potentiated by nobiletin. Furthermore, nobiletin stimulated lipolysis in differentiated adipocytes, which is known to be stimulated by cAMP pathway. These results suggested that nobiletin enhanced both differentiation and lipolysis of adipocyte through activation of signaling cascades mediated by cAMP/CREB.
Authors:
 [1] ;  [1] ;  [2]
  1. National Agricultural Research Center for Western Region, 1-3-1 Senyu-cho, Zentsuji 765-8508 (Japan)
  2. National Agricultural Research Center for Western Region, 1-3-1 Senyu-cho, Zentsuji 765-8508 (Japan). E-mail: ksekiya@affrc.go.jp
Publication Date:
OSTI Identifier:
20991363
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 357; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2007.03.169; PII: S0006-291X(07)00610-9; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMP; CITRUS; FRUITS; LIGANDS; LIPIDS; METABOLIC DISEASES; PHENOTYPE; POLYMERASE CHAIN REACTION; RECEPTORS; TRANSCRIPTION FACTORS