Biophysical characterization of V3-lipopeptide liposomes influencing HIV-1 infectivity
- Department of Chemistry and Foundation for Research and Technology-Hellas (FORTH-IESL), University of Crete, P.O. Box 2208, Heraklion 71003 (Greece)
- Department of Applied Biochemistry and Immunology, Institute of Molecular Biology and Biotechnology, FORTH, Heraklion, Crete (Greece)
- Department of Chemistry, Binghamton University, State University of New York, Binghamton, NY 13902-6000 (United States)
- Department of Virology, Medical School, University of Crete, Heraklion, Crete (Greece)
The V3-loop of the HIV-1 gp120 alters host cell immune function and modulates infectivity. We investigated biophysical parameters of liposome constructs with embedded lipopeptides from the principle neutralizing domain of the V3-loop and their influence on viral infectivity. Dynamic light scattering measurements showed liposome supramolecular structures with hydrodynamic radius of the order of 900 and 1300 nm for plain and V3-lipopeptide liposomes. Electron paramagnetic resonance measurements showed almost identical local microenvironment. The difference in liposome hydrodynamic radius was attributed to the fluctuating ionic environment of the V3-lipopeptide liposomes. In vitro HIV-1 infectivity assays showed that plain liposomes reduced virus production in all cell cultures, probably due to the hydrophobic nature of the aggregates. Liposomes carrying V3-lipopeptides with different cationic potentials restored and even enhanced infectivity (p < 0.05). These results highlight the need for elucidation of the involvement of lipid bilayers as dynamic components in supramolecular structures and in HIV-1 fusion mechanisms.
- OSTI ID:
- 20979885
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 355, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2007.02.052; PII: S0006-291X(07)00333-6; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Analyses of inter- and intra-patient variation in the V3 loop of the HIV-1 envelope protein
Plasma IgG to Linear Epitopes in the V2 and V3 Regions of HIV-1 gp120 Correlate with a Reduced Risk of Infection in the RV144 Vaccine Efficacy Trial