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Title: Induction of a program gene expression during osteoblast differentiation with strontium ranelate

Abstract

Strontium ranelate, a new agent for the treatment of osteoporosis, has been shown stimulate bone formation in various experimental models. This study examines the effect of strontium ranelate on gene expression in osteoblasts, as well as the formation of mineralized (von Kossa-positive) colony-forming unit-osteoblasts (CFU-obs). Bone marrow-derived stromal cells cultured for 21 days under differentiating conditions, when exposed to strontium ranelate, displayed a significant time- and concentration-dependent increase in the expression of the master gene, Runx2, as well as bone sialoprotein (BSP), but interestingly without effects on osteocalcin. This was associated with a significant increase in the formation of CFU-obs at day 21 of culture. In U-33 pre-osteoblastic cells, strontium ranelate significantly enhanced the expression of Runx2 and osteocalcin, but not BSP. Late, more mature osteoblastic OB-6 cells showed significant elevations in BSP and osteocalcin, but with only minimal effects on Runx2. In conclusion, strontium ranelate stimulates osteoblast differentiation, but the induction of the program of gene expression appears to be cell type-specific. The increased osteoblastic differentiation is the likely basis underlying the therapeutic bone-forming actions of strontium ranelate.

Authors:
 [1];  [1];  [1];  [1];  [1];  [2];  [2];  [1];  [1]
  1. Mount Sinai Bone Program, Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029 (United States)
  2. IRIS-Servier, Paris (France)
Publication Date:
OSTI Identifier:
20979863
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 355; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2007.01.120; PII: S0006-291X(07)00046-0; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BONE MARROW; COLONY FORMING UNITS; CONNECTIVE TISSUE CELLS; GENES; OSTEOPOROSIS; SKELETON; STRONTIUM

Citation Formats

Lingling, Zhu, Zaidi, Samir, Yuanzhen, Peng, Hang, Zhou, Moonga, Baljit S, Blesius, Alexia, Dupin-Roger, Isabelle, Zaidi, Mone, and Li, Sun. Induction of a program gene expression during osteoblast differentiation with strontium ranelate. United States: N. p., 2007. Web.
Lingling, Zhu, Zaidi, Samir, Yuanzhen, Peng, Hang, Zhou, Moonga, Baljit S, Blesius, Alexia, Dupin-Roger, Isabelle, Zaidi, Mone, & Li, Sun. Induction of a program gene expression during osteoblast differentiation with strontium ranelate. United States.
Lingling, Zhu, Zaidi, Samir, Yuanzhen, Peng, Hang, Zhou, Moonga, Baljit S, Blesius, Alexia, Dupin-Roger, Isabelle, Zaidi, Mone, and Li, Sun. 2007. "Induction of a program gene expression during osteoblast differentiation with strontium ranelate". United States.
@article{osti_20979863,
title = {Induction of a program gene expression during osteoblast differentiation with strontium ranelate},
author = {Lingling, Zhu and Zaidi, Samir and Yuanzhen, Peng and Hang, Zhou and Moonga, Baljit S and Blesius, Alexia and Dupin-Roger, Isabelle and Zaidi, Mone and Li, Sun},
abstractNote = {Strontium ranelate, a new agent for the treatment of osteoporosis, has been shown stimulate bone formation in various experimental models. This study examines the effect of strontium ranelate on gene expression in osteoblasts, as well as the formation of mineralized (von Kossa-positive) colony-forming unit-osteoblasts (CFU-obs). Bone marrow-derived stromal cells cultured for 21 days under differentiating conditions, when exposed to strontium ranelate, displayed a significant time- and concentration-dependent increase in the expression of the master gene, Runx2, as well as bone sialoprotein (BSP), but interestingly without effects on osteocalcin. This was associated with a significant increase in the formation of CFU-obs at day 21 of culture. In U-33 pre-osteoblastic cells, strontium ranelate significantly enhanced the expression of Runx2 and osteocalcin, but not BSP. Late, more mature osteoblastic OB-6 cells showed significant elevations in BSP and osteocalcin, but with only minimal effects on Runx2. In conclusion, strontium ranelate stimulates osteoblast differentiation, but the induction of the program of gene expression appears to be cell type-specific. The increased osteoblastic differentiation is the likely basis underlying the therapeutic bone-forming actions of strontium ranelate.},
doi = {},
url = {https://www.osti.gov/biblio/20979863}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 355,
place = {United States},
year = {Fri Apr 06 00:00:00 EDT 2007},
month = {Fri Apr 06 00:00:00 EDT 2007}
}