Tributyltin and triphenyltin inhibit osteoclast differentiation through a retinoic acid receptor-dependent signaling pathway
Abstract
Organotin compounds, such as tributyltin (TBT) and triphenyltin (TPT), have been widely used in agriculture and industry. Although these compounds are known to have many toxic effects, including endocrine-disrupting effects, their effects on bone resorption are unknown. In this study, we investigated the effects of organotin compounds, such as monobutyltin (MBT), dibutyltin (DBT), TBT, and TPT, on osteoclast differentiation using mouse monocytic RAW264.7 cells. MBT and DBT had no effects, whereas TBT and TPT dose-dependently inhibited osteoclast differentiation at concentrations of 3-30 nM. Treatment with a retinoic acid receptor (RAR)-specific antagonist, Ro41-5253, restored the inhibition of osteoclastogenesis by TBT and TPT. TBT and TPT reduced receptor activator of nuclear factor-{kappa}B ligand (RANKL) induced nuclear factor of activated T cells (NFAT) c1 expression, and the reduction in NFATc1 expression was recovered by Ro41-5253. Our results suggest that TBT and TPT suppress osteoclastogenesis by inhibiting RANKL-induced NFATc1 expression via an RAR-dependent signaling pathway.
- Authors:
-
- Research Institute for Biological Functions, Chubu University, 1200 Matsumoto, Kasugai-shi, Aichi 488-8501 (Japan)
- Department of Biological Chemistry, Chubu University, 1200 Matsumoto, Kasugai-shi, Aichi 487-8501 (Japan)
- Faculty of Biomedical Engineering, Toin University of Yokohama, 1614 Kurogane, Aoba, Yokohama, Kanagawa 225-8502 (Japan)
- Publication Date:
- OSTI Identifier:
- 20979851
- Resource Type:
- Journal Article
- Journal Name:
- Biochemical and Biophysical Research Communications
- Additional Journal Information:
- Journal Volume: 355; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2006.12.237; PII: S0006-291X(06)02828-2; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; AGRICULTURE; INHIBITION; LIGANDS; MICE; ORGANOMETALLIC COMPOUNDS; RECEPTORS; RETINOIC ACID; SKELETON; TIN COMPOUNDS; TOXICITY
Citation Formats
Yonezawa, Takayuki, Hasegawa, Shin-ichi, Ahn, Jae-Yong, Cha, Byung-Yoon, Teruya, Toshiaki, Hagiwara, Hiromi, Nagai, Kazuo, Department of Biological Chemistry, Chubu University, 1200 Matsumoto, Kasugai-shi, Aichi 487-8501, Woo, Je-Tae, Department of Biological Chemistry, Chubu University, 1200 Matsumoto, Kasugai-shi, Aichi 487-8501, and chubu ac jp, E-mail: jwoo@isc. Tributyltin and triphenyltin inhibit osteoclast differentiation through a retinoic acid receptor-dependent signaling pathway. United States: N. p., 2007.
Web. doi:10.1016/j.bbrc.2006.12.237.
Yonezawa, Takayuki, Hasegawa, Shin-ichi, Ahn, Jae-Yong, Cha, Byung-Yoon, Teruya, Toshiaki, Hagiwara, Hiromi, Nagai, Kazuo, Department of Biological Chemistry, Chubu University, 1200 Matsumoto, Kasugai-shi, Aichi 487-8501, Woo, Je-Tae, Department of Biological Chemistry, Chubu University, 1200 Matsumoto, Kasugai-shi, Aichi 487-8501, & chubu ac jp, E-mail: jwoo@isc. Tributyltin and triphenyltin inhibit osteoclast differentiation through a retinoic acid receptor-dependent signaling pathway. United States. https://doi.org/10.1016/j.bbrc.2006.12.237
Yonezawa, Takayuki, Hasegawa, Shin-ichi, Ahn, Jae-Yong, Cha, Byung-Yoon, Teruya, Toshiaki, Hagiwara, Hiromi, Nagai, Kazuo, Department of Biological Chemistry, Chubu University, 1200 Matsumoto, Kasugai-shi, Aichi 487-8501, Woo, Je-Tae, Department of Biological Chemistry, Chubu University, 1200 Matsumoto, Kasugai-shi, Aichi 487-8501, and chubu ac jp, E-mail: jwoo@isc. 2007.
"Tributyltin and triphenyltin inhibit osteoclast differentiation through a retinoic acid receptor-dependent signaling pathway". United States. https://doi.org/10.1016/j.bbrc.2006.12.237.
@article{osti_20979851,
title = {Tributyltin and triphenyltin inhibit osteoclast differentiation through a retinoic acid receptor-dependent signaling pathway},
author = {Yonezawa, Takayuki and Hasegawa, Shin-ichi and Ahn, Jae-Yong and Cha, Byung-Yoon and Teruya, Toshiaki and Hagiwara, Hiromi and Nagai, Kazuo and Department of Biological Chemistry, Chubu University, 1200 Matsumoto, Kasugai-shi, Aichi 487-8501 and Woo, Je-Tae and Department of Biological Chemistry, Chubu University, 1200 Matsumoto, Kasugai-shi, Aichi 487-8501 and chubu ac jp, E-mail: jwoo@isc},
abstractNote = {Organotin compounds, such as tributyltin (TBT) and triphenyltin (TPT), have been widely used in agriculture and industry. Although these compounds are known to have many toxic effects, including endocrine-disrupting effects, their effects on bone resorption are unknown. In this study, we investigated the effects of organotin compounds, such as monobutyltin (MBT), dibutyltin (DBT), TBT, and TPT, on osteoclast differentiation using mouse monocytic RAW264.7 cells. MBT and DBT had no effects, whereas TBT and TPT dose-dependently inhibited osteoclast differentiation at concentrations of 3-30 nM. Treatment with a retinoic acid receptor (RAR)-specific antagonist, Ro41-5253, restored the inhibition of osteoclastogenesis by TBT and TPT. TBT and TPT reduced receptor activator of nuclear factor-{kappa}B ligand (RANKL) induced nuclear factor of activated T cells (NFAT) c1 expression, and the reduction in NFATc1 expression was recovered by Ro41-5253. Our results suggest that TBT and TPT suppress osteoclastogenesis by inhibiting RANKL-induced NFATc1 expression via an RAR-dependent signaling pathway.},
doi = {10.1016/j.bbrc.2006.12.237},
url = {https://www.osti.gov/biblio/20979851},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 355,
place = {United States},
year = {Fri Mar 30 00:00:00 EDT 2007},
month = {Fri Mar 30 00:00:00 EDT 2007}
}