Bone marrow-derived osteoblast progenitor cells in circulating blood contribute to ectopic bone formation in mice

Journal Article · Fri Mar 09 00:00:00 EST 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.12.226· OSTI ID:20979828

Otsuru, Satoru ^[1]; Tamai, Katsuto ^[1]; Yamazaki, Takehiko ^[1]; Yoshikawa, Hideki ^[2]; Kaneda, Yasufumi ^[1]

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1. Division of Gene Therapy Science, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871 (Japan)
2. Department of Orthopaedic Surgery, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871 (Japan)

Recent studies have suggested the existence of osteoblastic cells in the circulation, but the origin and role of these cells in vivo are not clear. Here, we examined how these cells contribute to osteogenesis in a bone morphogenetic protein (BMP)-induced model of ectopic bone formation. Following lethal dose-irradiation and subsequent green fluorescent protein-transgenic bone marrow cell-transplantation (GFP-BMT) in mice, a BMP-2-containing collagen pellet was implanted into muscle. Three weeks later, a significant number of GFP-positive osteoblastic cells were present in the newly generated ectopic bone. Moreover, peripheral blood mononuclear cells (PBMNCs) from the BMP-2-implanted mouse were then shown to include osteoblast progenitor cells (OPCs) in culture. Passive transfer of the PBMNCs isolated from the BMP-2-implanted GFP-mouse to the BMP-2-implanted nude mouse led to GFP-positive osteoblast accumulation in the ectopic bone. These data provide new insight into the mechanism of ectopic bone formation involving bone marrow-derived OPCs in circulating blood.

• OSTI ID: 20979828
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 354, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2006.12.226; PII: S0006-291X(07)00006-X; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
BLOOD
BONE MARROW
BONE MARROW CELLS
COLLAGEN
EOSIN
FLUORESCEIN
HEMATOXYLIN
IN VIVO
IRRADIATION
ISOTHIOCYANATES
LETHAL DOSES
MICE
SKELETON