skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Aldehyde oxidase 1 is highly abundant in hepatic steatosis and is downregulated by adiponectin and fenofibric acid in hepatocytes in vitro

Abstract

Adiponectin protects the liver from steatosis caused by obesity or alcohol and therefore the influence of adiponectin on human hepatocytes was analyzed. GeneChip experiments indicated that recombinant adiponectin downregulates aldehyde oxidase 1 (AOX1) expression and this was confirmed by real-time RT-PCR and immunoblot. AOX1 is a xenobiotic metabolizing protein and produces reactive oxygen species (ROS), that promote cell damage and fibrogenesis. Adiponectin and fenofibric acid activate peroxisome proliferator-activated receptor-{alpha} (PPAR-{alpha}) and both suppress AOX1 protein and this is blocked by the PPAR-{alpha} antagonist RU486. Obesity is associated with low adiponectin, reduced hepatic PPAR-{alpha} activity and fatty liver, and AOX1 was found induced in the liver of rats on a high-fat diet when compared to controls. Free fatty acids and leptin, that are elevated in obesity, failed to upregulate AOX1 in vitro. The current data indicate that adiponectin reduces AOX1 by activating PPAR-{alpha} whereas fatty liver disease is associated with elevated hepatic AOX1. High AOX1 may be associated with higher ROS well described to induce fibrogenesis in liver tissue but may also influence drug metabolism and activity.

Authors:
 [1];  [1];  [1];  [2];  [1];  [1];  [1];  [3];  [1];  [1]
  1. Department of Internal Medicine I, University of Regensburg, D-93042 Regensburg (Germany)
  2. Center for Liver Cell Research, University of Regensburg (Germany)
  3. Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg (Germany)
Publication Date:
OSTI Identifier:
20854589
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 350; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2006.09.101; PII: S0006-291X(06)02158-9; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ALCOHOLS; ALDEHYDES; CARBOXYLIC ACIDS; DIET; DRUGS; FATS; IN VITRO; LEPTIN; LIVER; LIVER CELLS; METABOLIC DISEASES; METABOLISM; OXIDASES; POLYMERASE CHAIN REACTION; RATS; RECEPTORS

Citation Formats

Neumeier, Markus, Weigert, Johanna, Schaeffler, Andreas, Weiss, Thomas S, Department of Surgery, University of Regensburg, Schmidl, Christian, Buettner, Roland, Bollheimer, Cornelius, Aslanidis, Charalampos, Schoelmerich, Juergen, and Buechler, Christa. Aldehyde oxidase 1 is highly abundant in hepatic steatosis and is downregulated by adiponectin and fenofibric acid in hepatocytes in vitro. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2006.09.101.
Neumeier, Markus, Weigert, Johanna, Schaeffler, Andreas, Weiss, Thomas S, Department of Surgery, University of Regensburg, Schmidl, Christian, Buettner, Roland, Bollheimer, Cornelius, Aslanidis, Charalampos, Schoelmerich, Juergen, & Buechler, Christa. Aldehyde oxidase 1 is highly abundant in hepatic steatosis and is downregulated by adiponectin and fenofibric acid in hepatocytes in vitro. United States. https://doi.org/10.1016/j.bbrc.2006.09.101
Neumeier, Markus, Weigert, Johanna, Schaeffler, Andreas, Weiss, Thomas S, Department of Surgery, University of Regensburg, Schmidl, Christian, Buettner, Roland, Bollheimer, Cornelius, Aslanidis, Charalampos, Schoelmerich, Juergen, and Buechler, Christa. 2006. "Aldehyde oxidase 1 is highly abundant in hepatic steatosis and is downregulated by adiponectin and fenofibric acid in hepatocytes in vitro". United States. https://doi.org/10.1016/j.bbrc.2006.09.101.
@article{osti_20854589,
title = {Aldehyde oxidase 1 is highly abundant in hepatic steatosis and is downregulated by adiponectin and fenofibric acid in hepatocytes in vitro},
author = {Neumeier, Markus and Weigert, Johanna and Schaeffler, Andreas and Weiss, Thomas S and Department of Surgery, University of Regensburg and Schmidl, Christian and Buettner, Roland and Bollheimer, Cornelius and Aslanidis, Charalampos and Schoelmerich, Juergen and Buechler, Christa},
abstractNote = {Adiponectin protects the liver from steatosis caused by obesity or alcohol and therefore the influence of adiponectin on human hepatocytes was analyzed. GeneChip experiments indicated that recombinant adiponectin downregulates aldehyde oxidase 1 (AOX1) expression and this was confirmed by real-time RT-PCR and immunoblot. AOX1 is a xenobiotic metabolizing protein and produces reactive oxygen species (ROS), that promote cell damage and fibrogenesis. Adiponectin and fenofibric acid activate peroxisome proliferator-activated receptor-{alpha} (PPAR-{alpha}) and both suppress AOX1 protein and this is blocked by the PPAR-{alpha} antagonist RU486. Obesity is associated with low adiponectin, reduced hepatic PPAR-{alpha} activity and fatty liver, and AOX1 was found induced in the liver of rats on a high-fat diet when compared to controls. Free fatty acids and leptin, that are elevated in obesity, failed to upregulate AOX1 in vitro. The current data indicate that adiponectin reduces AOX1 by activating PPAR-{alpha} whereas fatty liver disease is associated with elevated hepatic AOX1. High AOX1 may be associated with higher ROS well described to induce fibrogenesis in liver tissue but may also influence drug metabolism and activity.},
doi = {10.1016/j.bbrc.2006.09.101},
url = {https://www.osti.gov/biblio/20854589}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 350,
place = {United States},
year = {Fri Nov 24 00:00:00 EST 2006},
month = {Fri Nov 24 00:00:00 EST 2006}
}