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Title: Analyzing the cellular contribution of bone marrow to fracture healing using bone marrow transplantation in mice

The bone marrow is believed to play important roles during fracture healing such as providing progenitor cells for inflammation, matrix remodeling, and cartilage and bone formation. Given the complex nature of bone repair, it remains difficult to distinguish the contributions of various cell types. Here we describe a mouse model based on bone marrow transplantation and genetic labeling to track cells originating from bone marrow during fracture healing. Following lethal irradiation and engraftment of bone marrow expressing the LacZ transgene constitutively, wild type mice underwent tibial fracture. Donor bone marrow-derived cells, which originated from the hematopoietic compartment, did not participate in the chondrogenic and osteogenic lineages during fracture healing. Instead, the donor bone marrow contributed to inflammatory and bone resorbing cells. This model can be exploited in the future to investigate the role of inflammation and matrix remodeling during bone repair, independent from osteogenesis and chondrogenesis.
Authors:
 [1] ;  [2] ;  [3]
  1. University of California at San Francisco, Department of Orthopaedic Surgery, San Francisco General Hospital, San Francisco, CA 94110 (United States). E-mail: colnotc@orthosurg.ucsf.edu
  2. University of California at San Francisco, Department of Orthopaedic Surgery, San Francisco General Hospital, San Francisco, CA 94110 (United States)
  3. Stanford University, 257 Campus Drive, Stanford, CA 94306 (United States)
Publication Date:
OSTI Identifier:
20854580
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 350; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2006.09.079; PII: S0006-291X(06)02112-7; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BIOLOGICAL RADIATION EFFECTS; BONE FRACTURES; BONE MARROW; CARTILAGE; CONNECTIVE TISSUE CELLS; GALACTOSIDASE; HEALING; INFLAMMATION; LETHAL IRRADIATION; MICE; REPAIR; SKELETON