SNAP-25 in hippocampal CA3 region is required for long-term memory formation
- Laboratory of Higher Brain Functions, Institute of Neurobiology, Institutes of Brain Science, Fudan University, 220 Han-Dan Road, Shanghai 200433 (China)
- Key Laboratory of Stem Cell Biology, Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031 (China)
- Research Center of Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 500 Cao-Bao Road, Shanghai 200233 (China)
- Chinese National Human Genome Center at Shanghai, 351 Guo-Shou-Jing Road, Zhang-Jiang Hi-Tech Park, Shanghai 201203 (China)
- Department of Genetics and Center of Alcohol Studies, Rutgers University, 145 Bevier Road, Piscataway, New Jersey 08854 (United States)
SNAP-25 is a synaptosomal protein of 25 kDa, a key component of synaptic vesicle-docking/fusion machinery, and plays a critical role in exocytosis and neurotransmitter release. We previously reported that SNAP-25 in the hippocampal CA1 region is involved in consolidation of contextual fear memory and water-maze spatial memory (Hou et al. European J Neuroscience, 20: 1593-1603, 2004). SNAP-25 is expressed not only in the CA1 region, but also in the CA3 region, and the SNAP-25 mRNA level in the CA3 region is higher than in the CA1 region. Here, we provide evidence that SNAP-25 in the CA3 region is also involved in learning/memory. Intra-CA3 infusion of SNAP-25 antisense oligonucleotide impaired both long-term contextual fear memory and water-maze spatial memory, with short-term memory intact. Furthermore, the SNAP-25 antisense oligonucleotide suppressed the long-term potentiation (LTP) of field excitatory post-synaptic potential (fEPSP) in the mossy-fiber pathway (DG-CA3 pathway), with no effect on paired-pulse facilitation of the fEPSP. These results are consistent with the notion that SNAP-25 in the hippocampal CA3 region is required for long-term memory formation.
- OSTI ID:
- 20854448
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 347, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2006.06.184; PII: S0006-291X(06)01490-2; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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