skip to main content

Title: C3d enhanced DNA vaccination induced humoral immune response to glycoprotein C of pseudorabies virus

Murine C3d were utilized to enhance immunogenicity of pseudorabies virus (PrV) gC DNA vaccination. Three copies of C3d and four copies of CR2-binding domain M28{sub 4} were fused, respectively, to truncated gC gene encoding soluble glycoprotein C (sgC) in pcDNA3.1. BALB/c mice were, respectively, immunized with recombinant plasmids, blank vector, and inactivated vaccine. The antibody ELISA titer for sgC-C3d{sub 3} DNA was 49-fold more than that for sgC DNA, and the neutralizing antibody obtained 8-fold rise. Protection of mice from death after lethal PrV (316 LD{sub 5}) challenge was augmented from 25% to 100%. Furthermore, C3d fusion increased Th2-biased immune response by inducing IL-4 production. The IL-4 level for sgC-C3d{sub 3} DNA immunization approached that for the inactivated vaccine. Compared to C3d, M28 enhanced sgC DNA immunogenicity to a lesser extent. In conclusion, we demonstrated that murine C3d fusion significantly enhanced gC DNA immunity by directing Th1-biased to a balanced and more effective Th1/Th2 response.
Authors:
 [1] ;  [2] ;  [1] ;  [2] ;  [1] ;  [2] ;  [1] ;  [2] ;  [1] ;  [1] ;  [2] ;  [3]
  1. National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070 (China)
  2. (China)
  3. National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070 (China) and Provincial Key Laboratory of Preventive Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070 (China). E-mail: aizhen@mail.hzau.edu.cn
Publication Date:
OSTI Identifier:
20854444
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 347; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2006.05.091; PII: S0006-291X(06)01148-X; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; ANTIGENS; DNA; ENZYME IMMUNOASSAY; GENES; GLYCOPROTEINS; IMMUNITY; MICE; PLASMIDS; VACCINES; VIRUSES