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Title: Rho GTPase activity modulates paramyxovirus fusion protein-mediated cell-cell fusion

The paramyxovirus fusion protein (F) promotes fusion of the viral envelope with the plasma membrane of target cells as well as cell-cell fusion. The plasma membrane is closely associated with the actin cytoskeleton, but the role of actin dynamics in paramyxovirus F-mediated membrane fusion is unclear. We examined cell-cell fusion promoted by two different paramyxovirus F proteins in three cell types in the presence of constitutively active Rho family GTPases, major cellular coordinators of actin dynamics. Reporter gene and syncytia assays demonstrated that expression of either Rac1{sup V12} or Cdc42{sup V12} could increase cell-cell fusion promoted by the Hendra or SV5 glycoproteins, though the effect was dependent on the cell type expressing the viral glycoproteins. In contrast, RhoA{sup L63} decreased cell-cell fusion promoted by Hendra glycoproteins but had little affect on SV5 F-mediated fusion. Also, data suggested that GTPase activation in the viral glycoprotein-containing cell was primarily responsible for changes in fusion. Additionally, we found that activated Cdc42 promoted nuclear rearrangement in syncytia.
Authors:
 [1] ;  [1] ;  [2] ;  [2] ;  [1] ;  [1] ;  [3]
  1. Department of Molecular and Cellular Biochemistry, University of Kentucky, 741 South Limestone, Lexington, KY 40536-0509 (United States)
  2. Department of Microbiology, Immunology and Molecular Genetics, UCLA, Los Angeles, CA 90095 (United States)
  3. Department of Molecular and Cellular Biochemistry, University of Kentucky, 741 South Limestone, Lexington, KY 40536-0509 (United States). E-mail: rdutc2@uky.edu
Publication Date:
OSTI Identifier:
20850525
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 350; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2006.01.033; PII: S0042-6822(06)00062-6; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACTIN; GENES; GLYCOPROTEINS; MEMBRANES; MICROTUBULES