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Title: Stability of membrane potential in heart mitochondria: Single mitochondrion imaging

Mitochondrial membrane potential ({delta}{psi} {sub m}) plays an important role in cellular activity. Although {delta}{psi} {sub m} of intracellular mitochondria are relatively stable, the recent experiments with isolated mitochondria demonstrate that individual mitochondria show frequent fluctuations of {delta}{psi} {sub m}. The current study is performed to investigate the factors that stabilize {delta}{psi} {sub m} in cells by observing {delta}{psi} {sub m} of individual isolated mitochondria with fluorescence microscopy. Here, we report that (1) the transient depolarizations are also induced for mitochondria in plasma membrane permeabilized cells, (2) almost all mitochondria isolated from porcine hearts show the transient depolarizations that is enhanced with the net efflux of protons from the matrix to the intermembrane space, and (3) ATP and ADP significantly inhibit the transient depolarizations by plural mechanisms. These results suggest that the suppression of acute alkalinization of the matrix together with the presence of ATP and ADP contributes to the stabilization of {delta}{psi} {sub m} in cells.
Authors:
 [1] ;  [1] ;  [1] ;  [2]
  1. Division of Biotechnology and Life Science, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology, Nakacho, Koganei, Tokyo 184-8588 (Japan)
  2. Division of Biotechnology and Life Science, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology, Nakacho, Koganei, Tokyo 184-8588 (Japan). E-mail: ohta@cc.tuat.ac.jp
Publication Date:
OSTI Identifier:
20798998
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 344; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2006.03.233; PII: S0006-291X(06)00781-9; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADP; ATP; DEPOLARIZATION; FLUORESCENCE; HEART; MICROSCOPY; MITOCHONDRIA; PROTONS