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Title: In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter

Abstract

We find that salicylic acid and ethephon treatment in hot pepper increases the expression of a putative basic/leucine zipper (bZIP) transcription factor gene, CabZIP1. CabZIP1 mRNA is expressed ubiquitously in various organs. The green fluorescent protein-fused transcription factor, CabZIP1::GFP, can be specifically localized to the nucleus, an action that is consistent with the presence of a nuclear localization signal in its protein sequence. Transient overexpression of the CabZIP1 transcription factor results in an increase in PR-1 transcripts level in Nicotiana benthamiana leaves. Using chromatin immunoprecipitation, we demonstrate that CabZIP1 binds to the G-box elements in native promoter of the hot pepper pathogenesis-related protein 1 (CaPR-1) gene in vivo. Taken together, our results suggest that CabZIP1 plays a role as a transcriptional regulator of the CaPR-1 gene.

Authors:
 [1];  [2];  [1];  [1];  [1]
  1. School of Life Sciences and Biotechnology, Korea University, 1, 5-ga, Anam-dong, Sungbuk-gu, Seoul 136-701 (Korea, Republic of)
  2. Department of Plant Pathology, University of California, Davis, CA 95616 (United States)
Publication Date:
OSTI Identifier:
20798960
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 344; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2006.03.153; PII: S0006-291X(06)00693-0; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMINO ACID SEQUENCE; CAPSICUM; CHROMATIN; GENES; IN VIVO; LEUCINE; NICOTIANA; ORGANS; PATHOGENESIS; PEPPERS; PROMOTERS; SALICYLIC ACID; TRANSCRIPTION FACTORS

Citation Formats

Lee, Boo-Ja, Park, Chang-Jin, Kim, Sung-Kyu, Kim, Ki-Jeong, and Paek, Kyung-Hee. In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2006.03.153.
Lee, Boo-Ja, Park, Chang-Jin, Kim, Sung-Kyu, Kim, Ki-Jeong, & Paek, Kyung-Hee. In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter. United States. https://doi.org/10.1016/j.bbrc.2006.03.153
Lee, Boo-Ja, Park, Chang-Jin, Kim, Sung-Kyu, Kim, Ki-Jeong, and Paek, Kyung-Hee. 2006. "In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter". United States. https://doi.org/10.1016/j.bbrc.2006.03.153.
@article{osti_20798960,
title = {In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter},
author = {Lee, Boo-Ja and Park, Chang-Jin and Kim, Sung-Kyu and Kim, Ki-Jeong and Paek, Kyung-Hee},
abstractNote = {We find that salicylic acid and ethephon treatment in hot pepper increases the expression of a putative basic/leucine zipper (bZIP) transcription factor gene, CabZIP1. CabZIP1 mRNA is expressed ubiquitously in various organs. The green fluorescent protein-fused transcription factor, CabZIP1::GFP, can be specifically localized to the nucleus, an action that is consistent with the presence of a nuclear localization signal in its protein sequence. Transient overexpression of the CabZIP1 transcription factor results in an increase in PR-1 transcripts level in Nicotiana benthamiana leaves. Using chromatin immunoprecipitation, we demonstrate that CabZIP1 binds to the G-box elements in native promoter of the hot pepper pathogenesis-related protein 1 (CaPR-1) gene in vivo. Taken together, our results suggest that CabZIP1 plays a role as a transcriptional regulator of the CaPR-1 gene.},
doi = {10.1016/j.bbrc.2006.03.153},
url = {https://www.osti.gov/biblio/20798960}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 344,
place = {United States},
year = {Fri May 26 00:00:00 EDT 2006},
month = {Fri May 26 00:00:00 EDT 2006}
}