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Title: SGT, a Hsp90{beta} binding partner, is accumulated in the nucleus during cell apoptosis

Abstract

In this study, we reported that small glutamine-rich TPR-containing protein (SGT) interacted with not only Hsp90{alpha} but also Hsp90{beta}. Confocal analysis showed that treatment of cells with Hsp90-specific inhibitor geldanamycin (GA) disrupted the interaction of SGT with Hsp90{beta} and this contributed to the increase of nuclear localization of SGT in HeLa cells. The increased nuclear localization of SGT was further confirmed by the Western blotting in GA-treated HeLa cells and H1299 cells. In our previous study, SGT was found to be a new pro-apoptotic factor, so we wondered whether the sub-cellular localization of SGT was related with cell apoptosis. By confocal analysis we found that the nuclear import of SGT was significantly increased in STS-induced apoptotic HeLa cells, which implied that the sub-cellular localization of SGT was closely associated with Hsp90{beta} and apoptosis.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1]; ;  [1];  [1]
  1. Key Laboratory of Medical Molecular Virology Ministry of Education and Health, Gene Research Center, Shanghai Medical College and Institutes of Biomedical Sciences of Fudan University, Shanghai 200032 (China)
Publication Date:
OSTI Identifier:
20798955
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 343; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2006.03.090; PII: S0006-291X(06)00619-X; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; CELL NUCLEI; GLUTAMINE; HELA CELLS; PROTEINS

Citation Formats

Hongyan, Yin, Hanzhou, Wang, Hongliang, Zong, Xiaoning, Chen, Yanlin, Wang, Xiaojing, Yun, Yihong, Wu, Jiadong, Wang, and Jianxin, Gu. SGT, a Hsp90{beta} binding partner, is accumulated in the nucleus during cell apoptosis. United States: N. p., 2006. Web.
Hongyan, Yin, Hanzhou, Wang, Hongliang, Zong, Xiaoning, Chen, Yanlin, Wang, Xiaojing, Yun, Yihong, Wu, Jiadong, Wang, & Jianxin, Gu. SGT, a Hsp90{beta} binding partner, is accumulated in the nucleus during cell apoptosis. United States.
Hongyan, Yin, Hanzhou, Wang, Hongliang, Zong, Xiaoning, Chen, Yanlin, Wang, Xiaojing, Yun, Yihong, Wu, Jiadong, Wang, and Jianxin, Gu. 2006. "SGT, a Hsp90{beta} binding partner, is accumulated in the nucleus during cell apoptosis". United States.
@article{osti_20798955,
title = {SGT, a Hsp90{beta} binding partner, is accumulated in the nucleus during cell apoptosis},
author = {Hongyan, Yin and Hanzhou, Wang and Hongliang, Zong and Xiaoning, Chen and Yanlin, Wang and Xiaojing, Yun and Yihong, Wu and Jiadong, Wang and Jianxin, Gu},
abstractNote = {In this study, we reported that small glutamine-rich TPR-containing protein (SGT) interacted with not only Hsp90{alpha} but also Hsp90{beta}. Confocal analysis showed that treatment of cells with Hsp90-specific inhibitor geldanamycin (GA) disrupted the interaction of SGT with Hsp90{beta} and this contributed to the increase of nuclear localization of SGT in HeLa cells. The increased nuclear localization of SGT was further confirmed by the Western blotting in GA-treated HeLa cells and H1299 cells. In our previous study, SGT was found to be a new pro-apoptotic factor, so we wondered whether the sub-cellular localization of SGT was related with cell apoptosis. By confocal analysis we found that the nuclear import of SGT was significantly increased in STS-induced apoptotic HeLa cells, which implied that the sub-cellular localization of SGT was closely associated with Hsp90{beta} and apoptosis.},
doi = {},
url = {https://www.osti.gov/biblio/20798955}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 343,
place = {United States},
year = {Fri May 19 00:00:00 EDT 2006},
month = {Fri May 19 00:00:00 EDT 2006}
}