Identification of a nuclear localization signal in the retinitis pigmentosa-mutated RP26 protein, ceramide kinase-like protein

Inagaki, Yuichi; Mitsutake, Susumu; Igarashi, Yasuyuki

doi:10.1016/j.bbrc.2006.03.056

Title: Identification of a nuclear localization signal in the retinitis pigmentosa-mutated RP26 protein, ceramide kinase-like protein

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Abstract

Retinitis pigmentosa (RP) is a genetically heterogeneous disease characterized by degeneration of the retina. A mutation in a new ceramide kinase (CERK) homologous gene, named CERK-like protein (CERKL), was found to cause autosomal recessive retinitis pigmentosa (RP26). Here, we show a point mutation of one of two putative nuclear localization signal (NLS) sequences inhibited the nuclear localization of the protein. Furthermore, the tetra-GFP-tagged NLS, which cannot passively enter the nucleus, was observed not only in the nucleus but also in the nucleolus. Our results provide First evidence of the active nuclear import of CERKL and suggest that the identified NLS might be responsible for nucleolar retention of the protein. As recent studies have shown other RP-related proteins are localized in the nucleus or the nucleolus, our identification of NLS in CERKL suggests that CERKL likely plays important roles for retinal functions in the nucleus and the nucleolus.

Authors:

Inagaki, Yuichi ^[1]; Mitsutake, Susumu ^[1]; Igarashi, Yasuyuki ^[1]

Department of Biomembrane and Biofunctional Chemistry, Graduate School of Pharmaceutical Science, Hokkaido University, Kita 12, Nishi 6, Kita-ku, Sapporo 060-0812 (Japan)

Publication Date:: Fri May 12 00:00:00 EDT 2006

OSTI Identifier:: 20798948

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 343; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2006.03.056; PII: S0006-291X(06)00561-4; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; CELL NUCLEI; GENE MUTATIONS; HEREDITARY DISEASES; PHOSPHATES; PROTEINS; RETENTION; RETINA

Citation Formats


                    Inagaki, Yuichi, Mitsutake, Susumu, and Igarashi, Yasuyuki. Identification of a nuclear localization signal in the retinitis pigmentosa-mutated RP26 protein, ceramide kinase-like protein.  United States: N. p., 2006. 
        Web.  doi:10.1016/j.bbrc.2006.03.056.

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                    Inagaki, Yuichi, Mitsutake, Susumu, & Igarashi, Yasuyuki. Identification of a nuclear localization signal in the retinitis pigmentosa-mutated RP26 protein, ceramide kinase-like protein.  United States.  https://doi.org/10.1016/j.bbrc.2006.03.056

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                    Inagaki, Yuichi, Mitsutake, Susumu, and Igarashi, Yasuyuki. 2006.  
        "Identification of a nuclear localization signal in the retinitis pigmentosa-mutated RP26 protein, ceramide kinase-like protein".  United States.  https://doi.org/10.1016/j.bbrc.2006.03.056.

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@article{osti_20798948,

  title        = {Identification of a nuclear localization signal in the retinitis pigmentosa-mutated RP26 protein, ceramide kinase-like protein},

  author       = {Inagaki, Yuichi and Mitsutake, Susumu and Igarashi, Yasuyuki},

  abstractNote = {Retinitis pigmentosa (RP) is a genetically heterogeneous disease characterized by degeneration of the retina. A mutation in a new ceramide kinase (CERK) homologous gene, named CERK-like protein (CERKL), was found to cause autosomal recessive retinitis pigmentosa (RP26). Here, we show a point mutation of one of two putative nuclear localization signal (NLS) sequences inhibited the nuclear localization of the protein. Furthermore, the tetra-GFP-tagged NLS, which cannot passively enter the nucleus, was observed not only in the nucleus but also in the nucleolus. Our results provide First evidence of the active nuclear import of CERKL and suggest that the identified NLS might be responsible for nucleolar retention of the protein. As recent studies have shown other RP-related proteins are localized in the nucleus or the nucleolus, our identification of NLS in CERKL suggests that CERKL likely plays important roles for retinal functions in the nucleus and the nucleolus.},

  doi          = {10.1016/j.bbrc.2006.03.056},

  url          = {https://www.osti.gov/biblio/20798948},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 3,

  volume       = 343,

  place        = {United States},

  year         = {Fri May 12 00:00:00 EDT 2006},

  month        = {Fri May 12 00:00:00 EDT 2006}

}

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