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Title: Mitogenic and chondrogenic effects of fibroblast growth factor-2 in adipose-derived mesenchymal cells

Adipose-derived mesenchymal cells (AMCs) have demonstrated a great capacity for differentiating into bone, cartilage, and fat. Studies using bone marrow-derived mesenchymal cells (BMSCs) have shown that fibroblast growth factor (FGF)-2, a potent mitogenic factor, plays an important role in tissue engineering due to its effects in proliferation and differentiation for mesenchymal cells. The aim of this study was to investigate the function of FGF-2 in AMC chondrogenic differentiation and its possible contributions to cell-based therapeutics in skeletal tissue regeneration. Data demonstrated that FGF-2 significantly promoted the proliferation of AMCs and enhanced chondrogenesis in three-dimensional micromass culture. Moreover, priming AMCs with treatment of FGF-2 at 10 ng/ml demonstrated that cells underwent chondrogenic phenotypic differentiation, possibly by inducing N-Cadherin, FGF-receptor 2, and transcription factor Sox9. Our results indicated that FGF-2 potentiates chondrogenesis in AMCs, similar to its functions in BMSCs, suggesting the versatile potential applications of FGF-2 in skeletal regeneration and cartilage repair.
Authors:
 [1] ;  [1] ;  [2]
  1. Children's Surgical Research Program, Department of Surgery, Stanford University School of Medicine (United States)
  2. Children's Surgical Research Program, Department of Surgery, Stanford University School of Medicine (United States). E-mail: Longaker@stanford.edu
Publication Date:
OSTI Identifier:
20798939
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 343; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2006.02.171; PII: S0006-291X(06)00431-1; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BONE MARROW; CARTILAGE; CELL PROLIFERATION; FIBROBLASTS; GROWTH FACTORS; RECEPTORS; REPAIR; SKELETON; TRANSCRIPTION FACTORS