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Title: The vitamin-like dietary supplement para-aminobenzoic acid enhances the antitumor activity of ionizing radiation

Purpose: To determine whether para-aminobenzoic acid (PABA) alters the sensitivity of tumor cells to ionizing radiation in vitro and in vivo. Methods and Materials: Cellular proliferation was assessed by WST-1 assays. The effects of PABA and radiation on tumor growth were examined with chick embryo and murine models. Real-time reverse transcriptase-polymerase chain reaction and Western blotting were used to quantify p21{sup CIP1} and CDC25A levels. Results: Para-aminobenzoic acid enhanced (by 50%) the growth inhibitory activity of radiation on B16F10 cells, whereas it had no effect on melanocytes. Para-aminobenzoic acid enhanced (50-80%) the antitumor activity of radiation on B16F10 and 4T1 tumors in vivo. The combination of PABA and radiation therapy increased tumor apoptosis. Treatment of tumor cells with PABA increased expression of CDC25A and decreased levels of p21{sup CIP1}. Conclusions: Our findings suggest that PABA might represent a compound capable of enhancing the antitumor activity of ionizing radiation by a mechanism involving altered expression of proteins known to regulate cell cycle arrest.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [2]
  1. Departments of Radiation Oncology and Cell Biology, NYU Cancer Institute, New York University School of Medicine, New York, NY (United States)
  2. Departments of Radiation Oncology and Cell Biology, NYU Cancer Institute, New York University School of Medicine, New York, NY (United States). E-mail: peter.brooks@med.nyu.edu
Publication Date:
OSTI Identifier:
20793513
Resource Type:
Journal Article
Resource Relation:
Journal Name: International Journal of Radiation Oncology, Biology and Physics; Journal Volume: 65; Journal Issue: 2; Other Information: DOI: 10.1016/j.ijrobp.2006.01.010; PII: S0360-3016(06)00108-8; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; APOPTOSIS; CELL CYCLE; CELL PROLIFERATION; EMBRYOS; GROWTH; IN VITRO; IN VIVO; MELANIN; NEOPLASMS; PABA; POLYMERASE CHAIN REACTION; PROTEINS; RADIOTHERAPY; SENSITIVITY; TUMOR CELLS; VITAMINS