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Title: Human T lymphocytes express N-methyl-D-aspartate receptors functionally active in controlling T cell activation

The aim of this study was to investigate the expression and the functional role of N-methyl-D-aspartate (NMDA) receptors in human T cells. RT-PCR analysis showed that human resting peripheral blood lymphocytes (PBL) and Jurkat T cells express genes encoding for both NR1 and NR2B subunits: phytohemagglutinin (PHA)-activated PBL also expresses both these genes and the NR2A and NR2D genes. Cytofluorimetric analysis showed that NR1 expression increases as a consequence of PHA (10 {mu}g/ml) treatment. D-(-)-2-Amino-5-phosphonopentanoic acid (D-AP5), and (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine [(+)-MK 801], competitive and non-competitive NMDA receptor antagonists, respectively, inhibited PHA-induced T cell proliferation, whereas they did not affect IL-2 (10 U/ml)-induced proliferation of PHA blasts. These effects were due to the prevention of T cell activation (inhibition of cell aggregate formation and CD25 expression), but not to cell cycle arrest or death. These results demonstrate that human T lymphocytes express NMDA receptors, which are functionally active in controlling cell activation.
Authors:
 [1] ;  [1] ;  [2]
  1. DiSCAFF Department, Eastern Piedmont University, Via Bovio 6, 28100 Novara (Italy)
  2. DiSCAFF Department, Eastern Piedmont University, Via Bovio 6, 28100 Novara (Italy). E-mail: lombardi@pharm.unipmn.it
Publication Date:
OSTI Identifier:
20793245
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 338; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2005.10.164; PII: S0006-291X(05)02444-7; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL CYCLE; CELL PROLIFERATION; DEATH; GENES; INHIBITION; LYMPHOCYTES; PHYTOHEMAGGLUTININ; POLYMERASE CHAIN REACTION; RECEPTORS