Alternative protein secretion: The Mam1 ABC transporter supports secretion of M-factor linked GFP in fission yeast
- Pharmexa A/S, Kogle Alle 6, DK-2970 Horsholm (Denmark)
To examine whether the fission yeast Mam1 ABC transporter can be used for secretion of heterologous proteins, thereby bypassing the classical secretion pathway, we have analyzed chimeric forms of the M-factor precursor. It was demonstrated that GFP can be exported when fused to both the amino-terminal prosequence from mfm1 and a CaaX motif. This secretion was dependent on the Mam1 transporter and not the classical secretion pathway. The secretion efficiency of GFP, however, was relatively low and most of the reporter protein was trapped in the vacuolar membranes. Our findings suggest that the Mam1 ABC protein is a promiscuous peptide transporter that can accommodate globular proteins of a relatively large size. Furthermore, our results help in defining the sequences required for processing and secretion of natural M-factor.
- OSTI ID:
- 20793244
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 338, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2005.10.156; PII: S0006-291X(05)02412-5; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Expression and secretion of fungal endoglucanase II and chimeric cellobiohydrolase I in the oleaginous yeast Lipomyces starkeyi
Comparison of different signal peptides for secretion of heterologous proteins in fission yeast