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Title: Induction of immune response in macaque monkeys infected with simian-human immunodeficiency virus having the TNF-{alpha} gene at an early stage of infection

TNF-{alpha} has been implicated in the pathogenesis of, and the immune response against, HIV-1 infection. To clarify the roles of TNF-{alpha} against HIV-1-related virus infection in an SHIV-macaque model, we genetically engineered an SHIV to express the TNF-{alpha} gene (SHIV-TNF) and characterized the virus's properties in vivo. After the acute viremic stage, the plasma viral loads declined earlier in the SHIV-TNF-inoculated monkeys than in the parental SHIV (SHIV-NI)-inoculated monkeys. SHIV-TNF induced cell death in the lymph nodes without depletion of circulating CD4{sup +} T cells. SHIV-TNF provided some immunity in monkeys by increasing the production of the chemokine RANTES and by inducing an antigen-specific proliferation of lymphocytes. The monkeys immunized with SHIV-TNF were partly protected against a pathogenic SHIV (SHIV-C2/1) challenge. These findings suggest that TNF-{alpha} contributes to the induction of an effective immune response against HIV-1 rather than to the progression of disease at the early stage of infection.
Authors:
 [1] ;  [2] ;  [2] ;  [2] ;  [2] ;  [1] ;  [2] ;  [2] ;  [3]
  1. Department of Veterinary Microbiology, University of Miyazaki, Miyazaki 889-2192 (Japan)
  2. Laboratory of Primate Model, Experimental Research Center for Infectious Disease, Institute for Virus Research, Kyoto University, Kyoto 606-8507 (Japan)
  3. Department of Veterinary Microbiology, University of Miyazaki, Miyazaki 889-2192 (Japan). E-mail: a0d518u@cc.miyazaki-u.ac.jp
Publication Date:
OSTI Identifier:
20779440
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 343; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2005.08.025; PII: S0042-6822(05)00515-5; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AIDS VIRUS; APOPTOSIS; CELL PROLIFERATION; GENES; IMMUNITY; IN VIVO; LYMPH NODES; LYMPHOCYTES; LYMPHOKINES; MONKEYS; PATHOGENESIS