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Title: Sample holder for small-angle x-ray scattering static and flow cell measurements

We present the design of a sample holder for small-angle x-ray scattering (SAXS) that can be used for both static and flow cell measurements, allowing to switch between these two types of measurement without having to realign the detector and camera geometry. The device makes possible high signal-to-noise experiments with sample volumes as small as 16 {mu}l and can be thermocontrolled using a standard circulating water bath. The setup has been used successfully for a range of biological SAXS measurements, including peptides, detergent micelles, membrane proteins, and nucleic acids. As a performance test, we present scattering data for horse heart cytochrome c, collected at the BESSRC CAT beam line 12-ID of the Advanced Photon Source. The design drawings are provided in the supplementary material.
Authors:
; ; ;  [1] ;  [2] ;  [2] ;  [2]
  1. Department of Physics, Geballe Laboratory of Advanced Materials, Stanford University, Stanford, California 94305 (United States)
  2. (United States)
Publication Date:
OSTI Identifier:
20779216
Resource Type:
Journal Article
Resource Relation:
Journal Name: Review of Scientific Instruments; Journal Volume: 77; Journal Issue: 4; Other Information: DOI: 10.1063/1.2194484; (c) 2006 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; ADVANCED PHOTON SOURCE; CAT SCANNING; DESIGN; HEART; HORSES; MEMBRANE PROTEINS; NUCLEIC ACIDS; SAMPLE HOLDERS; SMALL ANGLE SCATTERING; X-RAY DIFFRACTION