Interleukin-4 improves the migration of human myogenic precursor cells in vitro and in vivo

Journal Article · Sat Apr 15 00:00:00 EDT 2006 · Experimental Cell Research

DOI:https://doi.org/10.1016/j.yexcr.2006.01.002· OSTI ID:20775360

Lafreniere, J F ^[1]; Mills, P ^[1]; Bouchentouf, M ^[1]; Tremblay, J P ^[1]

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1. Unite de recherche en Genetique humaine, Centre Hospitalier de l'Universite Laval, 2705, boul. Laurier, RC-9300, Sainte-Foy (Quebec), G1V 4G2 (Canada)

Different molecules are available to recruit new neighboring myogenic cells to the site of regeneration. Formerly called B cell stimulatory factor-1, IL-4 can now be included in the list of motogenic factors. The present report demonstrates that human IL-4 is not required for fusion between mononucleated myoblasts but is required for myotube maturation. In identifying IL-4 as a pro-migratory agent for myogenic cells, these results provide a mechanism which partly explains IL-4 demonstrated activity during differentiation. Among the different mechanisms by which IL-4 might enhance myoblast migration processes, our results indicate that there are implications of some integrins and of three major components of the fibrinolytic system. Indeed, increases in the amount of active urokinase plasminogen activator and its receptor were observed following an IL-4 treatment, while the plasminogen activator inhibitor-1 decreased. Finally, IL-4 did not modify the amount of cell surface {alpha}5 integrin but increased the presence of {beta}3 and {beta}1 integrins. This integrin modulation might favor myogenic cell migration and its interaction with newly formed myotubes. Therefore, IL-4 co-injection with transplanted myoblasts might be an approach to enhance the migration of transplanted cells for the treatment of a damaged myocardium or of a Duchenne Muscular Dystrophy patient.

• OSTI ID: 20775360
• Journal Information: Experimental Cell Research, Vol. 312, Issue 7; Other Information: DOI: 10.1016/j.yexcr.2006.01.002; PII: S0014-4827(06)00004-8; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
IN VITRO
IN VIVO
MYOBLASTS
MYOCARDIUM
PATIENTS
PLASMINOGEN
PRECURSOR
RECEPTORS
UROKINASE