Nuclear localisation of endogenous SUMO-1-modified PDGF-C in human thyroid tissue and cell lines
- Department of Molecular Biology, University of Bergen, Section of Surgery, Haukeland University Hospital, University of Bergen, Postbox 7800, Bergen 5020 (Norway)
- Department of Biomedicine, University of Bergen, Bergen (Norway)
- Department of Surgical Sciences, Section of Surgery, Haukeland University Hospital, University of Bergen (Norway)
We investigated post-translational modification and subcellular localisation of endogenous platelet-derived growth factor-C (PDGF-C) in human thyroid papillary carcinomas (PTC), non-neoplastic thyroid tissues, and a selection of cultured cell lines. PDGF-C expressed nuclear localisation in 95% of all tested cell types in culture and in 10% of the thyrocytes from both PTC and non-neoplastic tissue. The cell lines expressed two forms of full-length PDGF-C, {approx}39 and {approx}55 kDa, in cell membrane and cytosol, while the {approx}55 kDa form dominated in the nucleus where it was partly chromatin-associated. The {approx}55 kDa form was post-translationally modified by SUMO-1. The putative PDGF-C SUMOylation site is the surface exposed {sup 314}lysine part of a positively charged loop ({sup 312}RPKTGVRGLHK{sup 322}) with characteristics of a nuclear localisation signal. The tissue thyrocytes expressed a non-SUMOylated {approx}43 kDa and the 55 kDa PDGF-C. The SUMO-1 modified {approx}55 kDa PDGF-C expression was low in PTC where the {approx}43 kDa PDGF-C dominated. This is in contrast to non-neoplastic tissue and cultured cells where the SUMOylated {approx}55 kDa PDGF-C was strongly expressed. Our data provide novel evidence for nuclear localisation of PDGF-C, post-translational modification by SUMOylation and the expression of a novel form of PDGF-C in human papillary thyroid carcinomas.
- OSTI ID:
- 20775346
- Journal Information:
- Experimental Cell Research, Vol. 312, Issue 6; Other Information: DOI: 10.1016/j.yexcr.2005.11.035; PII: S0014-4827(05)00564-1; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
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