Synthesis, cellular localization, and quantification of penton-dodecahedron in serotype 3 adenovirus-infected cells
- Institut de Biologie Structurale, 41, rue Horowitz, 38027 Grenoble (France)
- Centre Hospitalier Universitaire, BP217, 38043 La Tronche (France)
Adenovirus penton is a non-covalent complex composed of the penton base and fiber proteins, localized at the twelve vertices of the icosahedral virus capsid. In cells infected by adenovirus serotype 3 (Ad3), penton is found not only in the virus capsid but also self-assembled in dodecahedra formed through interactions between the twelve penton bases. In this study, the intracellular trafficking of penton proteins from the cytoplasm to the nucleus has been followed, and the nuclear re-arrangement induced by viral infection has been observed by electron microscopy of ultrathin sections. The amount of dodecahedra has been assessed in relation to the number of Ad3 infectious virions produced during the Ad3 replication cycle. It appears that dodecahedra are produced in a large excess over viral infectious particles and that they are located intranuclearly along the nuclear membrane of Ad3-infected cells at late times of infection.
- OSTI ID:
- 20729133
- Journal Information:
- Virology, Vol. 340, Issue 2; Other Information: DOI: 10.1016/j.virol.2005.06.030; PII: S0042-6822(05)00361-2; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
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