Early BrdU-responsive genes constitute a novel class of senescence-associated genes in human cells
- Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City University, Maioka-cho 641-12, Yokohama 244-0813 (Japan)
- Genetics and Genomic Biology Program, Hospital for Sick Children and Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, M5G 1X8 (Canada)
We identified genes that immediately respond to 5-bromodeoxyuridine (BrdU) in SUSM-1, an immortal fibroblastic line, with DNA microarray and Northern blot analysis. At least 29 genes were found to alter gene expression greater than twice more or less than controls within 36 h after addition of BrdU. They took several different expression patterns upon addition of BrdU, and the majority showed a significant alteration within 12 h. When compared among SUSM-1, HeLa, and TIG-7 normal human fibroblasts, 19 genes behaved similarly upon addition of BrdU. In addition, 14 genes, 9 of which are novel as regards senescence, behaved similarly in senescent TIG-7 cells. The genes do not seem to have a role in proliferation or cell cycle progression. These results suggest that the early BrdU-responsive genes represent early signs of cellular senescence and can be its new biomarkers.
- OSTI ID:
- 20717569
- Journal Information:
- Experimental Cell Research, Vol. 304, Issue 2; Other Information: DOI: 10.1016/j.yexcr.2004.10.036; PII: S0014-4827(04)00650-0; Copyright (c) 2004 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
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