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Title: Anti-apoptotic role of retinoic acid in the inner ear of noise-exposed mice

Exposure to loud noise can induce temporary or permanent hearing loss, and acoustic trauma is the major cause of hearing impairment in industrial nations. However, the mechanisms underlying the death of hair cells after acoustic trauma remain unclear. In addition to its involvement in cellular stress and apoptosis, the c-Jun N-terminal kinase (JNK), a member of the mitogen-activated protein kinase family, is involved in cell survival, transformation, embryonic morphogenesis, and differentiation. JNK is primarily activated by various environmental stresses including noise, and the phenotypic result appears be to cell death. All-trans retinoic acid (ATRA) is an active metabolite of vitamin A that regulates a wide range of biological processes, including cell proliferation, differentiation, and morphogenesis. We evaluated the role of ATRA in preserving hearing in mice exposed to noise that can induce permanent hearing loss. Mice fed with ATRA before and during 3 consecutive days of noise exposure had a more preserved hearing threshold than mice fed sesame oil or saline. Histological and TUNEL staining of the cochlea showed significantly enhanced preservation of the organ of Corti, including outer hair cells and relatively low apoptotic nuclei, in mice-fed ATRA than in mice-fed sesame oil or saline. Phospho-JNK immunohistochemistry showed thatmore » ATRA inhibited the activation of JNK. These results suggest that ATRA has an anti-apoptotic effect on cochleae exposed to noise.« less
Authors:
 [1] ;  [2] ;  [1] ;  [3]
  1. Department of Otolaryngology, Asan Medical Center, University of Ulsan, College of Medicine, Seoul (Korea, Republic of)
  2. Asan Institute for Life Sciences, Seoul (Korea, Republic of)
  3. Department of Otolaryngology, Asan Medical Center, University of Ulsan, College of Medicine, Seoul (Korea, Republic of). E-mail: jwchung@amc.seoul.kr
Publication Date:
OSTI Identifier:
20710982
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 335; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2005.07.114; PII: S0006-291X(05)01589-5; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; AUDITORY ORGANS; BIOLOGICAL STRESS; CELL PROLIFERATION; HAIR; INJURIES; MICE; MORPHOGENESIS; NOISE; PROTEINS; RETINOIC ACID; SESAME OIL; VITAMIN A