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Title: Identification and characterization of a selenium-dependent glutathione peroxidase in Setaria cervi

Setaria cervi a bovine filarial parasite secretes selenium glutathione peroxidase during in vitro cultivation. A significant amount of enzyme activity was detected in the somatic extract of different developmental stages of the parasite. Among different stages, microfilariae showed a higher level of selenium glutathione peroxidase activity followed by males then females. However, when the activity was compared in excretory secretory products of these stages males showed higher activity than microfilariae and female worms. The enzyme was purified from female somatic extract using a combination of glutathione agarose and gel filtration chromatography, which migrated as a single band of molecular mass {approx}20 kDa. Selenium content of purified enzyme was estimated by atomic absorption spectroscopy and found to be 3.5 ng selenium/{mu}g of protein. Further, inhibition of enzyme activity by potassium cyanide suggested the presence of selenium at the active site of enzyme. This is the first report of identification of selenium glutathione peroxidase from any filarial parasite.
Authors:
 [1] ;  [2]
  1. Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, UP (India)
  2. Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, UP (India). E-mail: sushmarathaur@yahoo.com
Publication Date:
OSTI Identifier:
20710801
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 331; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2005.03.235; PII: S0006-291X(05)00743-6; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ABSORPTION SPECTROSCOPY; CATTLE; CHROMATOGRAPHY; CYANIDES; ENZYME ACTIVITY; GLUTATHIONE; IN VITRO; PARASITES; PEROXIDASES; POTASSIUM; SELENIUM