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Title: Intraepithelial lymphocytes express junctional molecules in murine small intestine

Intestinal intraepithelial lymphocytes (IEL) that reside at basolateral site regulate the proliferation and differentiation of epithelial cells (EC) for providing a first line of host defense in intestine. However, it remains unknown how IEL interact and communicate with EC. Here, we show that IEL express junctional molecules like EC. We identified mRNA expression of the junctional molecules in IEL such as zonula occludens (ZO)-1, occludin and junctional adhesion molecule (JAM) (tight junction), {beta}-catenin and E-cadherin (adherens junction), and connexin26 (gap junction). IEL constitutively expressed occludin and E-cadherin at protein level, while other T cells in the thymus, spleen, liver, mesenteric lymph node, and Peyer's patches did not. {gamma}{delta} IEL showed higher level of these expressions than {alpha}{beta} IEL. The expression of occludin was augmented by anti-CD3 Ab stimulation. These results suggest the possibility of a novel role of IEL concerning epithelial barrier and communication between IEL and EC.
Authors:
 [1] ;  [2] ;  [2] ;  [3] ;  [4]
  1. Parasitic Diseases Unit, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692 (Japan). E-mail: INAGAKI@med.miyazaki-u.ac.jp
  2. Department of Anatomy, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692 (Japan)
  3. Molecular Microbiology Group, Center of Molecular Biosciences, University of the Ryukyus, Senbaru, Nishihara, Okinawa 903-0213 (Japan)
  4. Parasitic Diseases Unit, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692 (Japan)
Publication Date:
OSTI Identifier:
20710794
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 331; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2005.04.025; PII: S0006-291X(05)00772-2; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; LIVER; LYMPH NODES; LYMPHOCYTES; PROTEINS; SMALL INTESTINE; SPLEEN; THYMUS