Preformed {beta}-amyloid fibrils are destabilized by coenzyme Q{sub 10} in vitro

Journal Article · Fri Apr 29 00:00:00 EDT 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.02.132· OSTI ID:20709159

Ono, Kenjiro ^[1]; Hasegawa, Kazuhiro ^[2]; Naiki, Hironobu ^[2]; Yamada, Masahito ^[1]

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1. Department of Neurology and Neurobiology of Aging, Kanazawa University Graduate School of Medical Science, Kanazawa 920-8640 (Japan)
2. Department of Pathology, Fukui University, Fukui 910-1193, CREST of Japan Science and Technology Corporation, Kawaguchi 332-0012 (Japan)

Inhibition of the formation of {beta}-amyloid fibrils (fA{beta}), as well as the destabilization of preformed fA{beta} in the CNS, would be attractive therapeutic targets for the treatment of Alzheimer's disease (AD). We reported previously that nordihydroguaiaretic acid (NDGA) and wine-related polyphenol, myricetin (Myr), inhibit fA{beta} formation from A{beta} and destabilize preformed fA{beta} in vitro. Using fluorescence spectroscopic analysis with thioflavin T and electron microscopic studies, we examined the effects of coenzyme Q{sub 10} (CoQ{sub 10}) on the formation, extension, and destabilization of fA{beta} at pH 7.5 at 37 deg C in vitro. We next compared the anti-amyloidogenic activities of CoQ{sub 10} with NDGA and Myr. CoQ{sub 10} dose-dependently inhibited fA{beta} formation from amyloid {beta}-peptide (A{beta}), as well as their extension. Moreover, it destabilized preformed fA{beta}s. The anti-amyloidogenic effects of CoQ{sub 10} were slightly weaker than those of NDGA and Myr. CoQ{sub 10} could be a key molecule for the development of therapeutics for AD.

• OSTI ID: 20709159
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 330, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2005.02.132; PII: S0006-291X(05)00406-7; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BEVERAGES
COENZYMES
ELECTRON MICROSCOPY
FLUORESCENCE
IN VITRO
MOLECULES
NERVOUS SYSTEM DISEASES
PEPTIDES
PH VALUE