Acute and chronic effects of exposure to a 1-mT magnetic field on the cytoskeleton, stress proteins, and proliferation of astroglial cells in culture
- Departamento de Biologia Celular y Genetica, Facultad de Biologia, Universidad de Alcala, 28871 Alcala de Henares, Madrid (Spain)
- Departamento de Biologia Celular, Facultad de Biologia, Universidad Complutense, 28040 Madrid (Spain)
This paper reports the effects of exposure to static, sinusoidal (50 Hz), and combined static/sinusoidal magnetic fields on cultured astroglial cells. Confluent primary cultures of astroglial cells were exposed to a 1-mT sinusoidal, static, or combined magnetic field for 1 h. In another experiment, cells were exposed to the combined magnetic field for 1, 2, and 4 h. The hsp25, hsp60, hsp70, actin, and glial fibrillary acidic protein contents of the astroglial cells were determined by immunoblotting 24 h after exposure. No significant differences were seen between control and exposed cells with respect to their contents of these proteins, neither were any changes in cell morphology observed. In a third experiment to determine the effect of a chronic (11-day) exposure to a combined 1-mT static/sinusoidal magnetic field on the proliferation of cultured astroglial cells, no significant differences were seen between control, sham-exposed, or exposed cells. These results suggest that exposure to 1-mT sinusoidal, static, or combined magnetic fields has no significant effects on the stress, cytoskeletal protein levels in, or proliferation of cultured astroglial cells.
- OSTI ID:
- 20696120
- Journal Information:
- Environmental Research, Vol. 98, Issue 3; Other Information: DOI: 10.1016/j.envres.2004.12.010; PII: S0013-9351(04)00247-6; Copyright (c) 2004 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0013-9351
- Country of Publication:
- United States
- Language:
- English
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