Hepatic progenitor cell resistance to TGF-{beta}1's proliferative and apoptotic effects

Clark, J Brian; Rice, Lisa; Sadiq, Tim; Brittain, Evan; Song, Lujun; Jian, Wang; Gerber, David A

doi:10.1016/j.bbrc.2005.01.129

Title: Hepatic progenitor cell resistance to TGF-{beta}1's proliferative and apoptotic effects

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Abstract

The success of hepatocellular therapies using stem or progenitor cell populations is dependent upon multiple factors including the donor cell, microenvironment, and etiology of the liver injury. The following experiments investigated the impact of TGF-{beta}1 on a previously described population of hepatic progenitor cells (HPC). The majority of the hepatic progenitor cells were resistant to endogenously produced TGF-{beta}1's proapoptotic and anti-proliferative effects unlike more well-differentiated cellular populations (e.g., mature hepatocytes). Surprisingly, in vitro TGF-{beta}1 supplementation significantly inhibited de novo hepatic progenitor cell colony formation possibly via an indirect mechanism(s). Therefore despite the HPC's direct resistance to supplemental TGF-{beta}1, this cytokine's inhibitory effect on colony formation could have a potential negative impact on the use of these cells as a therapy for patients with liver disease.

Authors:

Clark, J Brian ^[1]; Rice, Lisa ^[1]; Sadiq, Tim ^[1]; Brittain, Evan ^[1]; Song, Lujun ^[1]; Jian, Wang ^[1]; Gerber, David A ^[1]

CB 7211, 2111 Bioinformatics Building, Department of Surgery, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7211 (United States)

Publication Date:: Fri Apr 01 00:00:00 EST 2005

OSTI Identifier:: 20630913

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 329; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2005.01.129; PII: S0006-291X(05)00172-5; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; APOPTOSIS; COLONY FORMATION; ETIOLOGY; IN VITRO; INJURIES; LIVER; LIVER CELLS; LYMPHOKINES; PATIENTS; STEM CELLS; THERAPY

Citation Formats


                    Clark, J Brian, Rice, Lisa, Sadiq, Tim, Brittain, Evan, Song, Lujun, Jian, Wang, and Gerber, David A. Hepatic progenitor cell resistance to TGF-{beta}1's proliferative and apoptotic effects.  United States: N. p., 2005. 
        Web.  doi:10.1016/j.bbrc.2005.01.129.

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                    Clark, J Brian, Rice, Lisa, Sadiq, Tim, Brittain, Evan, Song, Lujun, Jian, Wang, & Gerber, David A. Hepatic progenitor cell resistance to TGF-{beta}1's proliferative and apoptotic effects.  United States.  https://doi.org/10.1016/j.bbrc.2005.01.129

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                    Clark, J Brian, Rice, Lisa, Sadiq, Tim, Brittain, Evan, Song, Lujun, Jian, Wang, and Gerber, David A. 2005.  
        "Hepatic progenitor cell resistance to TGF-{beta}1's proliferative and apoptotic effects".  United States.  https://doi.org/10.1016/j.bbrc.2005.01.129.

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@article{osti_20630913,

  title        = {Hepatic progenitor cell resistance to TGF-{beta}1's proliferative and apoptotic effects},

  author       = {Clark, J Brian and Rice, Lisa and Sadiq, Tim and Brittain, Evan and Song, Lujun and Jian, Wang and Gerber, David A},

  abstractNote = {The success of hepatocellular therapies using stem or progenitor cell populations is dependent upon multiple factors including the donor cell, microenvironment, and etiology of the liver injury. The following experiments investigated the impact of TGF-{beta}1 on a previously described population of hepatic progenitor cells (HPC). The majority of the hepatic progenitor cells were resistant to endogenously produced TGF-{beta}1's proapoptotic and anti-proliferative effects unlike more well-differentiated cellular populations (e.g., mature hepatocytes). Surprisingly, in vitro TGF-{beta}1 supplementation significantly inhibited de novo hepatic progenitor cell colony formation possibly via an indirect mechanism(s). Therefore despite the HPC's direct resistance to supplemental TGF-{beta}1, this cytokine's inhibitory effect on colony formation could have a potential negative impact on the use of these cells as a therapy for patients with liver disease.},

  doi          = {10.1016/j.bbrc.2005.01.129},

  url          = {https://www.osti.gov/biblio/20630913},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 1,

  volume       = 329,

  place        = {United States},

  year         = {Fri Apr 01 00:00:00 EST 2005},

  month        = {Fri Apr 01 00:00:00 EST 2005}

}

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