CerioFAST{trademark}: An acute toxicity test based on Ceriodaphnia dubia feeding behavior
- Univ. of Florida, Gainesville, FL (United States). Dept. of Environmental Engineering
The authors have developed a rapid acute toxicity test (CerioFAST{trademark}) based on suppression of feeding activity of Ceriodaphnia dubia in the presence of toxicants. The bioassay consists of a 1-h exposure period to a given toxicant. Yeast cells, stained with a fluorescent dye, are added 20 min before the end of the exposure period. Response to a toxic sample is indicated by the absence of fluorescence in the gut of the daphnids. CerioFAST was compared to the standard 48-h C. dubia acute bioassay, using heavy metals and organic compounds.CerioFAST EC50s of Cd, Cu, Pb, Ag, Zn, and carbofuran were in the 0.01--0.1-mg/L range, whereas EC50s of hexachloroethane, pentachlorophenol, trichlorophenol, and lindane were in the 1--10-mg/L range. CerioFAST EC50s of the heavy metals and organics were well correlated with Ec50s obtained with the 48-h C. dubia bioassay.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 201250
- Journal Information:
- Environmental Toxicology and Chemistry, Vol. 15, Issue 2; Other Information: PBD: Feb 1996
- Country of Publication:
- United States
- Language:
- English
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