Quantification of phnAc and nahAc in contaminated New Zealand soils by competitive PCR
Unculturable polycyclic aromatic hydrocarbon (PAH)-degrading bacteria are a significant reservoir of the microbial potential to catabolize low-molecular-weight PAHs. The population of these bacteria is larger than the population of nah-like bacteria that are the dominant organisms in culture-based studies. The authors used the recently described phn genes of Burkholderia sp. strain RP007, which feature only rarely in culture-based studies, as an alternative genotype for naphthalene and phenanthrene degradation and compared this genotype with the genotypically distinct but ubiquitous nah-like class in different soils. Competitive PCR quantification of phnAc and nahAc, which encode the iron sulfur protein large ({alpha}) subunits of PAH dioxygenases in nah-like and phn catabolic operons, revealed that the phn genotype can have a greater ecological significance than the nah-like genotype.
- Research Organization:
- Landcare Research, Hamilton (NZ)
- OSTI ID:
- 20075756
- Journal Information:
- Applied and Environmental Microbiology, Vol. 66, Issue 5; Other Information: PBD: May 2000; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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