Streptavidin mutants
The present invention relates to streptavidin proteins and peptides having a altered physical properties such as an increased stability or increased or decreased affinity for binding biotin. The invention also relates to methods for the detection, identification, separation and isolation of targets using streptavidin proteins or peptides. Streptavidin with increased or reduced affinity allows for the use of the streptavidin-biotin coupling systems for detection and isolation systems wherein it is necessary to remove of one or the other of the binding partners. Such systems are useful for the purification of functional proteins and viable cells. The invention also relates to nucleic acids which encode these streptavidin proteins and peptides and to recombinant cells such as bacteria, yeast and mammalian cells which contain these nucleic acids.
- Research Organization:
- US Department of Energy, Washington, DC (US)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG02-93ER61656
- Assignee:
- PTO; EDB-00:006690
- Application Number:
- Int. Cl. C07K 14/36; US 6,022,951/A/; 8-628,540; TRN: IM200010%%137
- OSTI ID:
- 20013269
- Resource Relation:
- Other Information: PBD: 8 Feb 2000
- Country of Publication:
- United States
- Language:
- English
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