The mechanism of synthesis of a mixed-linkage (1{r{underscore}arrow}3), (1{r{underscore}arrow}4) {beta}-D-glucan in maize. Evidence for multiple sites of glucosyl transfer in the synthase complex
The authors examined the mechanism of synthesis in vitro of (1{r{underscore}arrow}3), (1{r{underscore}arrow}4){Beta}-D-glucan ({Beta}-glucan), a growth-specific cell wall polysaccharide round in grasses and cereals. {beta}-Glucan is composed primarily of cellotriosyl and cellotetraosyl units linked by single (1{r{underscore}arrow}3){beta}-linkages. The ratio of cellotriosyl and cellotetraosyl units in the native polymer is strictly controlled at between 2 and 3 in all grasses, whereas the ratios of these units in {Beta}-glucan formed in vitro vary from 1.5 with 5 {micro}M UDP-glucose (GLc) to over 11 with 30 nM substrate. These results support a model in which three sites of glycosyl transfer occur within the synthase complex to produce the cellobiosyl-(1{r{underscore}arrow}3)-D-glucosyl units. The authors propose that failure to fill one of the sites results in the iterative addition of one or more cellobiosyl units to produce the longer cellodextrin units in the polymer. Variations in the UDP-Glc concentration in excised maize (Zea mays) coleoptiles did not result in wide variations in the ratios of cellotriosyl and cellotetraosyl units in {beta}-glucan synthesized in vivo, indicating that other factors control delivery of UDP-Glc to the synthase. In maize sucrose synthase is enriched in Golgi membranes and plasma membranes and may be involved in the control of substrate delivery to {beta}-glucan synthase and cellulose synthase.
- Research Organization:
- Inst. de Botanica, Sao Paulo (BR)
- OSTI ID:
- 20006196
- Journal Information:
- Plant Physiology (Bethesda), Vol. 120, Issue 4; Other Information: PBD: Aug 1999; ISSN 0032-0889
- Country of Publication:
- United States
- Language:
- English
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