Chemical analysis of individual mammalian cells
Abstract
The extremely small size of mammalian cells creates an unusual challenge for the analytical chemist, both in terms of separation and detection. Under a microscope, it is possible to confirm the injection of individual cells such as erythrocyte into capillaries with 10-{mu}m i.d. by hydrostatic pressure. The ionic contents can then be separated by capillary electrophoresis after the cell lyses. Enzymes at the zeptomole level can be monitored by on-column fluorescence enzyme assay. On-column particle-counting immunoassay can be applied to a broad range of analytes (antigens), also at the zeptomole level. The authors report here the simultaneous determination of the amounts of glucose-6-phosphate dehydrogenase (G6PDH) and their activities in individual erythrocytes by using a combination of the two detection schemes. Insights into the degradation of proteins as a function of cell age can be derived.
- Authors:
-
- Ames Lab., IA (United States)
- Publication Date:
- OSTI Identifier:
- 191665
- Report Number(s):
- CONF-941098-
TRN: 95:006733-0021
- Resource Type:
- Conference
- Resource Relation:
- Conference: FACSS XXI: 21st annual conference of the Federation of Analytical Chemistry and Spectroscopy Societies (FACSS), St. Louis, MO (United States), 2-7 Oct 1994; Other Information: PBD: 1994; Related Information: Is Part Of 21st annual conference of the Federation of Analytical Chemistry and Spectroscopy Societies; PB: 257 p.
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; 40 CHEMISTRY; ANIMAL CELLS; CHEMICAL ANALYSIS; ENZYMES; QUANTITATIVE CHEMICAL ANALYSIS; FLUORESCENCE SPECTROSCOPY; ERYTHROCYTES; ELECTROPHORESIS
Citation Formats
Tan, W, and Yeung, E S. Chemical analysis of individual mammalian cells. United States: N. p., 1994.
Web.
Tan, W, & Yeung, E S. Chemical analysis of individual mammalian cells. United States.
Tan, W, and Yeung, E S. 1994.
"Chemical analysis of individual mammalian cells". United States.
@article{osti_191665,
title = {Chemical analysis of individual mammalian cells},
author = {Tan, W and Yeung, E S},
abstractNote = {The extremely small size of mammalian cells creates an unusual challenge for the analytical chemist, both in terms of separation and detection. Under a microscope, it is possible to confirm the injection of individual cells such as erythrocyte into capillaries with 10-{mu}m i.d. by hydrostatic pressure. The ionic contents can then be separated by capillary electrophoresis after the cell lyses. Enzymes at the zeptomole level can be monitored by on-column fluorescence enzyme assay. On-column particle-counting immunoassay can be applied to a broad range of analytes (antigens), also at the zeptomole level. The authors report here the simultaneous determination of the amounts of glucose-6-phosphate dehydrogenase (G6PDH) and their activities in individual erythrocytes by using a combination of the two detection schemes. Insights into the degradation of proteins as a function of cell age can be derived.},
doi = {},
url = {https://www.osti.gov/biblio/191665},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Dec 31 00:00:00 EST 1994},
month = {Sat Dec 31 00:00:00 EST 1994}
}