Human {beta}-globin gene polymorphisms characterized in DNA extracted from ancient bones 12,000 years old
- Genetique Medicale et Developpement, Marseille (France)
- Institut Paoli-Calmettes, Marseille (France); and others
Analyzing the nuclear DNA from ancient human bones is an essential step to the understanding of genetic diversity in current populations, provided that such systematic studies are experimentally feasible. This article reports the successful extraction and amplification of nuclear DNA from the P-globin region from 5 of 10 bone specimens up to 12,000 years old. These have been typed for P-globin frameworks by sequencing through two variable positions and for a polymorphic (AT){sub x}(T){sub y} microsatellite 500 bp upstream of the P-globin gene. These specimens of human remains are somewhat older than those analyzed in previous nuclear gene sequencing reports and considerably older than those used to study high-copy-number human mtDNA. These results show that the systematic study of nuclear DNA polymorphisms of ancient populations is feasible. 34 refs., 3 figs., 2 tabs.
- OSTI ID:
- 186196
- Journal Information:
- American Journal of Human Genetics, Vol. 57, Issue 6; Other Information: PBD: Dec 1995
- Country of Publication:
- United States
- Language:
- English
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