An STS-based map of the human genome

Journal Article · Fri Dec 22 00:00:00 EST 1995 · Science

DOI:https://doi.org/10.1126/science.270.5244.1945· OSTI ID:181893

Hudson, T J; Stein, L D; Gerety, S S ^[1]

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1. and others

A physical map has been constructed of the human genome containing 15,086 sequence-tagged sites (STSs), with an average spacing of 199 kilobases. The project involved assembly of a radiation hybrid map of the human genome containing 5264 loci. This information was combined with the results of STS-content screening of 10,850 loci against a yeast artificial chromosome library to produce an integrated map, anchored by the radiation hybrid and genetic maps. The map provides radiation hybrid coverage of 99 percent and physical coverage of 94 percent of the human genome. The map also represents an early step in an international project to generate a transcript map of the human genome, with more than 3235 expressed sequences localized. The STSs in the map provide a scaffold for initiating large-scale sequencing of the human genome.

• Sponsoring Organization: USDOE
• OSTI ID: 181893
• Journal Information: Science, Vol. 270, Issue 5244; Other Information: PBD: 22 Dec 1995
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
GENETIC MAPPING
RESEARCH PROGRAMS
HUMAN POPULATIONS