Influence of developmental stage, salts and food presence on various end points using Caenorhabditis elegans for aquatic toxicity testing

Donkin, S G; Williams, P L

doi:10.1002/etc.5620141218

Title: Influence of developmental stage, salts and food presence on various end points using Caenorhabditis elegans for aquatic toxicity testing

Full Record
Other Related Research

Abstract

This study used a randomized block design to investigate the importance of several variables in using the free-living soil nematode, Caenorhabditis elegans, for aquatic toxicity testing. Concentration-response data were obtained on nematodes of various developmental stages exposed to four metals (Cd, Pb, Cu, and Hg) and a water-soluble organic toxicant, sodium pentachlorophenate (PCP), under conditions of varied solvent medium (with or without salts and with or without a bacterial food source). The end points measured were 24- and 96-h mortality LC50 value, as well as development of larval stages to adulthood and evidence of reproduction. The results suggest that nematodes of various ages respond similarity to a given toxicant for all end points measured, although adults cultured from eggs appeared more sensitive than adults cultured from dauer larvae. The most important environmental variable in determining toxicity was the medium in which the tests were conducted. The presence of potassium and sodium salts in the medium significantly (p < 0.05) reduced the toxicity of many test samples. The presence of bacteria had little effect on 24-h tests with salts, but was important in 96-h survival and development. Based on sensitivity and ease of handling, adults cultured from eggs are recommended inmore » both 24h and 96-h tests.« less

Authors:

Donkin, S G; Williams, P L ^[1]

Univ. of Georgia, Athens, GA (United States)

Publication Date:: Fri Dec 01 00:00:00 EST 1995

Sponsoring Org.:: USDOE

OSTI Identifier:: 162959

Resource Type:: Journal Article

Journal Name:: Environmental Toxicology and Chemistry

Additional Journal Information:: Journal Volume: 14; Journal Issue: 12; Other Information: PBD: Dec 1995

Country of Publication:: United States

Language:: English

Subject:: 54 ENVIRONMENTAL SCIENCES; 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; WATER POLLUTION; BIOLOGICAL INDICATORS; NEMATODES; SENSITIVITY; CADMIUM; TOXICITY; LEAD; COPPER; MERCURY; DOSE-RESPONSE RELATIONSHIPS; AGE DEPENDENCE

Citation Formats


                    Donkin, S G, and Williams, P L. Influence of developmental stage, salts and food presence on various end points using Caenorhabditis elegans for aquatic toxicity testing.  United States: N. p., 1995. 
        Web.  doi:10.1002/etc.5620141218.

Copy to clipboard


                    Donkin, S G, & Williams, P L. Influence of developmental stage, salts and food presence on various end points using Caenorhabditis elegans for aquatic toxicity testing.  United States.  https://doi.org/10.1002/etc.5620141218

Copy to clipboard


                    Donkin, S G, and Williams, P L. 1995.  
        "Influence of developmental stage, salts and food presence on various end points using Caenorhabditis elegans for aquatic toxicity testing".  United States.  https://doi.org/10.1002/etc.5620141218.

Copy to clipboard


                    
@article{osti_162959,

  title        = {Influence of developmental stage, salts and food presence on various end points using Caenorhabditis elegans for aquatic toxicity testing},

  author       = {Donkin, S G and Williams, P L},

  abstractNote = {This study used a randomized block design to investigate the importance of several variables in using the free-living soil nematode, Caenorhabditis elegans, for aquatic toxicity testing. Concentration-response data were obtained on nematodes of various developmental stages exposed to four metals (Cd, Pb, Cu, and Hg) and a water-soluble organic toxicant, sodium pentachlorophenate (PCP), under conditions of varied solvent medium (with or without salts and with or without a bacterial food source). The end points measured were 24- and 96-h mortality LC50 value, as well as development of larval stages to adulthood and evidence of reproduction. The results suggest that nematodes of various ages respond similarity to a given toxicant for all end points measured, although adults cultured from eggs appeared more sensitive than adults cultured from dauer larvae. The most important environmental variable in determining toxicity was the medium in which the tests were conducted. The presence of potassium and sodium salts in the medium significantly (p < 0.05) reduced the toxicity of many test samples. The presence of bacteria had little effect on 24-h tests with salts, but was important in 96-h survival and development. Based on sensitivity and ease of handling, adults cultured from eggs are recommended in both 24h and 96-h tests.},

  doi          = {10.1002/etc.5620141218},

  url          = {https://www.osti.gov/biblio/162959},
  journal      = {Environmental Toxicology and Chemistry},
number       = 12,

  volume       = 14,

  place        = {United States},

  year         = {Fri Dec 01 00:00:00 EST 1995},

  month        = {Fri Dec 01 00:00:00 EST 1995}

}

Copy to clipboard

Journal Article:

https://doi.org/10.1002/etc.5620141218

Other availability

Find in Google Scholar

Search WorldCat to find libraries that may hold this journal

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

Effects of developmental stage, salts, and food presence on aquatic toxicological endpoints using Caenorhabditis elegans

Conference Donkin, S; Williams, P

The objective of this study was to standardize the testing protocol for aquatic toxicity tests with the nematode Caenorhabditis elegans. Several variables which may be important in determining the test outcome were investigated in a randomized block design. Concentration-response data were obtained on nematodes of various developmental stages exposed to four metals (Cd, Pb, Cu, and Hg) and a water-soluble organic toxicant, sodium Pentachlorophenate (PCP), under conditions of varied solvent medium (with or without salts and with or without a bacterial food source). The endpoints measured were 24 and 96-h mortality, as well as development of larval stages to adulthoodmore »« less
Reference toxicants for toxicity testing using Caenorhabditis elegans in aquatic media

Conference Cressman, III, C; Williams, P

Caenorhabditis elegans aquatic toxicity assays were standardized with five common reference toxicants: CdCl{sub 2}, NaCl, KCl, sodium lauryl sulfate (SLS), and sodium pentachlorophenate (PCP). Aquatic toxicity testing was conducted in 3 media: a standard C. elegans medium; EPA moderately hard reconstituted water; and EPA moderately hard mineral water. Test duration in each medium was 24h without a food source, and 24h and 48h with Escherichia coli strain OP50 as a food source. Each test was replicated three times with each replicate having 6 wells per concentration, 10 worms per well. LC{sub 50} values were calculated using probit analysis. The averagemore »« less
The Phenotypic and Transcriptomic Response of the Caenorhabditis elegans Nematode to Background and Below-Background Radiation Levels

Journal Article Van Voorhies, Wayne; Castillo, Hugo; Thawng, Cung; ... - Frontiers in Public Health

Studies of the biological effects of low-level and below-background radiation are important in understanding the potential effects of radiation exposure in humans. To study this issue we exposed the nematode Caenorhabditis elegans to average background and below-background radiation levels. Two experiments were carried-out in the underground radiation biology laboratory at the Waste Isolation Pilot Plant (WIPP) in New Mexico USA. The first experiment used naïve nematodes with data collected within 1 week of being placed underground. The second experiment used worms that were incubated for 8 months underground at below background radiation levels. Nematode eggs were placed in two incubators,more »« less
https://doi.org/10.3389/fpubh.2020.581796
Biochemistry and molecular biology of the Caenorhabditis elegans dauer larva

Miscellaneous Wadsworth, W

Biochemical and molecular techniques have been used to study the formation and recovery of the developmentally arrested, non-feeding dauer stage of the nematode Caenorhabditis elegans. While investigating developmental transitions in energy metabolism, a major metabolite isolated from perchloric acid extracts has been identified as a modified uridine nucleotide. The compound was isolated by gel filtration and ion-exchange chromatography and its structure was determined by {sup 1}H NMR and {sup 13}C NMR spectroscopy. This compound is the most abundant metabolite detected in {sup 31}PMR spectra of perchloric acid extracts from growing larvae. In the absence of phosphoarginine or phosphocreatine, this modifiedmore »« less
A high-throughput method for assessing chemical toxicity using a Caenorhabditis elegans reproduction assay

Journal Article Boyd, Windy; McBride, Sandra; Rice, Julie; ... - Toxicology and Applied Pharmacology

The National Research Council has outlined the need for non-mammalian toxicological models to test the potential health effects of a large number of chemicals while also reducing the use of traditional animal models. The nematode Caenorhabditis elegans is an attractive alternative model because of its well-characterized and evolutionarily conserved biology, low cost, and ability to be used in high-throughput screening. A high-throughput method is described for quantifying the reproductive capacity of C. elegans exposed to chemicals for 48 h from the last larval stage (L4) to adulthood using a COPAS Biosort. Initially, the effects of exposure conditions that could influencemore »« less
https://doi.org/10.1016/j.taap.2010.02.014

Similar Records