Use of the electrically driven emulsion-phase contactor for a biphasic liquid-liquid enzyme system
- Oak Ridge National Lab., TN (United States); and others
An alternative approach to the operation of bioprocessing systems within non-aqueous environments would require the development of reaction systems that would provide effective interfacial contact between the biocatalyst, contained within an aqueous phase, and the organic phase containing the substrate. A biphasic liquid-liquid (BLL) reactor that provides for intimate liquid-liquid contact would be the most probable approach for this application. For the BLL reactions considered in this work, the overall effectiveness of the system will depend on both compatibility of the biocatalyst with the chemical species present and intrinsic reaction and interfacial transport phenomena typically involved with liquid-liquid operations. The focus of this article is to investigate the removal and oxidation of p-cresol dissolved in toluene by aqueous-phase horseradish peroxidase. Contacting of the liquid-liquid biphasic enzyme system is carried out in an advanced solvent extraction contacting device, the electrically driven emulsion-phase contactor (EPC).
- Research Organization:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 150415
- Report Number(s):
- CONF-940526-; TRN: 95:007973-0027
- Resource Relation:
- Conference: 16. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN (United States), 9-13 May 1994; Other Information: PBD: 1995; Related Information: Is Part Of Sixteenth symposium on biotechnology for fuels and chemicals; Davison, B.H. [ed.] [Oak Ridge National Lab., TN (United States)]; Wyman, C.E. [ed.] [National Renewable Energy Lab., Golden, CO (United States)]; PB: 823 p.
- Country of Publication:
- United States
- Language:
- English
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