Optimization of A Portable Microanalytical System to Reduce Electrode Fouling from Proteins Associated with Biomonitoring of Lead (Pb) in Saliva

Yantasee, Wassana; Timchalk, Chuck; Weitz, Karl K; Moore, Dean A; Lin, Yuehe

doi:10.1016/j.talanta.2005.03.039

Title: Optimization of A Portable Microanalytical System to Reduce Electrode Fouling from Proteins Associated with Biomonitoring of Lead (Pb) in Saliva

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Abstract

There is a need to develop reliable portable analytical systems for on-site and real-time biomonitoring of lead (Pb) from both occupational and environmental exposures. Saliva is an appealing matrix since it is easily obtainable, and therefore a potential substitute for blood since there is a reasonably good correlation between Pb levels in both blood and saliva. The microanalytical system is based on stripping voltammetry of Pb at the microelectrochemical cell having a flow injection/flow-onto design. Samples that contain as little as 1% saliva can cause electrode fouling, resulting in significantly reduced responsiveness, irreproducible quantitations, and the need for frequent electrode regeneration. In addition, incomplete Pb release from salivary protein can also yield a lower Pb response than expected. This paper evaluates the extent of in vitro Pb-protein binding and the optimal pre-treatment for releasing Pb from the saliva samples. Even in 50% by volume of rat saliva, the electrode fouling was not observed, due to the appropriate sample pretreatment (with 1.0 M acid, followed by centrifugation at the RCF of 15200?g) and the constant flow of the sample and acidic carrier that prevented passivation by the protein. The system offered a linear response over a low Pb range (1-10 ppb),more »« less

Authors:: Yantasee, Wassana; Timchalk, Chuck; Weitz, Karl K; Moore, Dean A; Lin, Yuehe

Publication Date:: Thu Sep 15 00:00:00 EDT 2005

Research Org.:: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)

Sponsoring Org.:: USDOE

OSTI Identifier:: 15020817

Report Number(s):: PNWD-SA-6834
11098; TRN: US200521%%369

DOE Contract Number:: AC05-76RL01830

Resource Type:: Journal Article

Journal Name:: Talanta

Additional Journal Information:: Journal Volume: 67; Journal Issue: 3

Country of Publication:: United States

Language:: English

Subject:: 36 MATERIALS SCIENCE; BLOOD; CENTRIFUGATION; DESIGN; ELECTRODES; ENVIRONMENTAL EXPOSURE; FOULING; IN VITRO; OPTIMIZATION; PASSIVATION; PROTEINS; REGENERATION; RELIABILITY; SALIVA; SENSITIVITY; environmental molecular sciences laboratory; lead, electrochemical sensor, saliva, protein, stripping voltammetry, electrode fouling, passivation

Citation Formats


                    Yantasee, Wassana, Timchalk, Chuck, Weitz, Karl K, Moore, Dean A, and Lin, Yuehe. Optimization of A Portable Microanalytical System to Reduce Electrode Fouling from Proteins Associated with Biomonitoring of Lead (Pb) in Saliva.  United States: N. p., 2005. 
        Web.  doi:10.1016/j.talanta.2005.03.039.

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                    Yantasee, Wassana, Timchalk, Chuck, Weitz, Karl K, Moore, Dean A, & Lin, Yuehe. Optimization of A Portable Microanalytical System to Reduce Electrode Fouling from Proteins Associated with Biomonitoring of Lead (Pb) in Saliva.  United States.  https://doi.org/10.1016/j.talanta.2005.03.039

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                    Yantasee, Wassana, Timchalk, Chuck, Weitz, Karl K, Moore, Dean A, and Lin, Yuehe. 2005.  
        "Optimization of A Portable Microanalytical System to Reduce Electrode Fouling from Proteins Associated with Biomonitoring of Lead (Pb) in Saliva".  United States.  https://doi.org/10.1016/j.talanta.2005.03.039.

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@article{osti_15020817,

  title        = {Optimization of A Portable Microanalytical System to Reduce Electrode Fouling from Proteins Associated with Biomonitoring of Lead (Pb) in Saliva},

  author       = {Yantasee, Wassana and Timchalk, Chuck and Weitz, Karl K and Moore, Dean A and Lin, Yuehe},

  abstractNote = {There is a need to develop reliable portable analytical systems for on-site and real-time biomonitoring of lead (Pb) from both occupational and environmental exposures. Saliva is an appealing matrix since it is easily obtainable, and therefore a potential substitute for blood since there is a reasonably good correlation between Pb levels in both blood and saliva. The microanalytical system is based on stripping voltammetry of Pb at the microelectrochemical cell having a flow injection/flow-onto design. Samples that contain as little as 1% saliva can cause electrode fouling, resulting in significantly reduced responsiveness, irreproducible quantitations, and the need for frequent electrode regeneration. In addition, incomplete Pb release from salivary protein can also yield a lower Pb response than expected. This paper evaluates the extent of in vitro Pb-protein binding and the optimal pre-treatment for releasing Pb from the saliva samples. Even in 50% by volume of rat saliva, the electrode fouling was not observed, due to the appropriate sample pretreatment (with 1.0 M acid, followed by centrifugation at the RCF of 15200?g) and the constant flow of the sample and acidic carrier that prevented passivation by the protein. The system offered a linear response over a low Pb range (1-10 ppb), low detection limit (1 ppb), excellent reproducibility (5% RSD), and reliability. It also yielded the same Pb concentrations in unknown samples as did the ICP-MS. These encouraging results suggest that the microanalytical system represents an important analytical advancement for real-time non-invasive (i.e., saliva) biomonitoring of Pb.},

  doi          = {10.1016/j.talanta.2005.03.039},

  url          = {https://www.osti.gov/biblio/15020817},
  journal      = {Talanta},
number       = 3,

  volume       = 67,

  place        = {United States},

  year         = {Thu Sep 15 00:00:00 EDT 2005},

  month        = {Thu Sep 15 00:00:00 EDT 2005}

}

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