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Title: Microsecond Microfluidic Mixing for Investigation of Protein Folding Kinetics

We have developed and characterized a mixer to study the reaction kinetics of protein folding on a microsecond timescale. The mixer uses hydrodynamic focusing of pressure-driven flow in a microfluidic channel to reduce diffusion times as first demonstrated by Knight et al.[1]. Features of the mixer include 1 {micro}s mixing times, sample consumptions of order 1 nl/s, loading sample volumes on the order of microliters, and the ability to manufacture in fused silica for compatibility with most spectroscopic methods.
Authors:
; ;
Publication Date:
OSTI Identifier:
15014683
Report Number(s):
UCRL-PROC-209712
TRN: US200807%%700
DOE Contract Number:
W-7405-ENG-48
Resource Type:
Conference
Resource Relation:
Conference: Presented at: Micro Total Analysis Systems, Squaw Valley, CA (US), 10/05/2003--10/09/2003; Other Information: PBD: 10 Feb 2005
Research Org:
Lawrence Livermore National Lab., Livermore, CA (US)
Sponsoring Org:
US Department of Energy (US)
Country of Publication:
United States
Language:
English
Subject:
42 ENGINEERING; 59 BASIC BIOLOGICAL SCIENCES; COMPATIBILITY; DIFFUSION; FOCUSING; HYDRODYNAMICS; KINETICS; MIXERS; PROTEINS; REACTION KINETICS; SILICA