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Title: Nanoscale Proteomics

Abstract

This paper describes efforts to develop a liquid chromatography (LC)/mass spectrometry (MS) technology for ultra-sensitive proteomics studies, i.e. nanoscale proteomics. The approach combines high-efficiency nano-scale LC with advanced MS, including high sensitivity and high resolution Fourier transform ion cyclotron resonance (FTICR) MS, to perform both single-stage MS and tandem MS (MS/MS) proteomic analyses. The technology developed enables large-scale protein identification from nanogram size proteomic samples and characterization of more abundant proteins from sub-picogram size complex samples. Protein identification in such studies using MS is feasible from <75 zeptomole of a protein, and the average proteome measurement throughput is >200 proteins/h and ~3 h/sample. Higher throughput (>1000 proteins/h) and more sensitive detection limits can be obtained using a “accurate mass and time” tag approach developed at our laboratory. These capabilities lay the foundation for studies from single or limited numbers of cells.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
15007488
Report Number(s):
PNNL-SA-39154
Journal ID: ISSN 1618-2642; KP1601010; TRN: US200419%%511
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Analytical and Bioanalytical Chemistry, 378(4):1037-1045
Additional Journal Information:
Journal Volume: 378; Journal Issue: 4; Journal ID: ISSN 1618-2642
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CHROMATOGRAPHY; ION CYCLOTRON-RESONANCE; PROTEINS; RESOLUTION; SENSITIVITY; SPECTROSCOPY

Citation Formats

Shen, Yufeng, Tolic, Nikola, Masselon, Christophe D, Pasa-Tolic, Liljiana, Camp, David G, Anderson, Gordon A, Smith, Richard D, and Lipton, Mary S. Nanoscale Proteomics. United States: N. p., 2004. Web. doi:10.1007/s00216-003-2329-8.
Shen, Yufeng, Tolic, Nikola, Masselon, Christophe D, Pasa-Tolic, Liljiana, Camp, David G, Anderson, Gordon A, Smith, Richard D, & Lipton, Mary S. Nanoscale Proteomics. United States. https://doi.org/10.1007/s00216-003-2329-8
Shen, Yufeng, Tolic, Nikola, Masselon, Christophe D, Pasa-Tolic, Liljiana, Camp, David G, Anderson, Gordon A, Smith, Richard D, and Lipton, Mary S. 2004. "Nanoscale Proteomics". United States. https://doi.org/10.1007/s00216-003-2329-8.
@article{osti_15007488,
title = {Nanoscale Proteomics},
author = {Shen, Yufeng and Tolic, Nikola and Masselon, Christophe D and Pasa-Tolic, Liljiana and Camp, David G and Anderson, Gordon A and Smith, Richard D and Lipton, Mary S},
abstractNote = {This paper describes efforts to develop a liquid chromatography (LC)/mass spectrometry (MS) technology for ultra-sensitive proteomics studies, i.e. nanoscale proteomics. The approach combines high-efficiency nano-scale LC with advanced MS, including high sensitivity and high resolution Fourier transform ion cyclotron resonance (FTICR) MS, to perform both single-stage MS and tandem MS (MS/MS) proteomic analyses. The technology developed enables large-scale protein identification from nanogram size proteomic samples and characterization of more abundant proteins from sub-picogram size complex samples. Protein identification in such studies using MS is feasible from <75 zeptomole of a protein, and the average proteome measurement throughput is >200 proteins/h and ~3 h/sample. Higher throughput (>1000 proteins/h) and more sensitive detection limits can be obtained using a “accurate mass and time” tag approach developed at our laboratory. These capabilities lay the foundation for studies from single or limited numbers of cells.},
doi = {10.1007/s00216-003-2329-8},
url = {https://www.osti.gov/biblio/15007488}, journal = {Analytical and Bioanalytical Chemistry, 378(4):1037-1045},
issn = {1618-2642},
number = 4,
volume = 378,
place = {United States},
year = {Sun Feb 01 00:00:00 EST 2004},
month = {Sun Feb 01 00:00:00 EST 2004}
}