Development of Next Generation Synthetic Biology Tools for Use in Streptomyces venezuelae

Phelan, Ryan M.; Sachs, Daniel; Petkiewicz, Shayne J.; Barajas, Jesus F.; Blake-Hedges, Jacquelyn M.; Thompson, Mitchell G.; Reider Apel, Amanda; Rasor, Blake J.; Katz, Leonard; Keasling, Jay D.

doi:10.1021/acssynbio.6b00202

Title: Development of Next Generation Synthetic Biology Tools for Use in Streptomyces venezuelae

Journal Article · Wed Sep 07 00:00:00 EDT 2016 · ACS Synthetic Biology

DOI:https://doi.org/10.1021/acssynbio.6b00202· OSTI ID:1393116

Phelan, Ryan M. ^[1]; Sachs, Daniel ^[2]; Petkiewicz, Shayne J. ^[2]; Barajas, Jesus F. ^[2]; Blake-Hedges, Jacquelyn M. ^[3]; Thompson, Mitchell G. ^[4]; Reider Apel, Amanda ^[2]; Rasor, Blake J. ^[5]; Katz, Leonard ^[6]; Keasling, Jay D. ^[7]

Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of California, Berkeley, CA (United States). QB3 Inst.
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
Univ. of California, Berkeley, CA (United States). Dept. of Chemistry
Univ. of California, Berkeley, CA (United States). Dept. of Plant & Microbial Biology
Miami Univ., Oxford, Ohio (United States). Dept. of Biology
Univ. of California, Berkeley, CA (United States). QB3 Inst.
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of California, Berkeley, CA (United States). QB3 Inst.; Univ. of California, Berkeley, CA (United States). Dept. of Chemical and Biomolecular Engineering and Department of Bioengineering; Technical Univ. of Denmark, Kogle Alle (Denmark). Novo Nordisk Foundation Center for Biosustainability

Streptomyces have a rich history as producers of important natural products and this genus of bacteria has recently garnered attention for its potential applications in the broader context of synthetic biology. However, the dearth of genetic tools available to control and monitor protein production precludes rapid and predictable metabolic engineering that is possible in hosts such as Escherichia coli or Saccharomyces cerevisiae. In an effort to improve genetic tools for Streptomyces venezuelae, we developed a suite of standardized, orthogonal integration vectors and an improved method to monitor protein production in this host. These tools were applied to characterize heterologous promoters and various attB chromosomal integration sites. A final study leveraged the characterized toolset to demonstrate its use in producing the biofuel precursor bisabolene using a chromosomally integrated expression system. In conclusion, these tools advance S. venezuelae to be a practical host for future metabolic engineering efforts.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Energy Efficiency and Renewable Energy (EERE); National Science Foundation (NSF)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1393116

Journal Information:: ACS Synthetic Biology, Vol. 6, Issue 1; ISSN 2161-5063

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 56 works

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Title: Development of Next Generation Synthetic Biology Tools for Use in Streptomyces venezuelae

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