skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Polymer microfilters with nanostructured surfaces for the culture of circulating cancer cells

Abstract

There is a critical need to improve the accuracy of drug screening and testing through the development of in vitro culture systems that more effectively mimic the in vivo environment. Surface topographical features on the nanoscale level, in short nanotopography, effect the cell growth patterns, and hence affect cell function in culture. We report the preliminary results on the fabrication, and subsequent cellular growth, of nanoscale surface topography on polymer microfilters using cell lines as a precursor to circulating tumor cells (CTCs). To create various nanoscale features on the microfilter surface, we used reactive ion etching (RIE) with and without an etching mask. An anodized aluminum oxide (AAO) membrane fabricated directly on the polymer surface served as an etching mask. Polymer filters with a variety of modified surfaces were used to compare the effects on the culture of cancer cell lines in blank culture wells, with untreated microfilters or with RIE-treated microfilters. We then report the differences of cell shape, phenotype and growth patterns of bladder and glioblastoma cancer cell lines after isolation on the various types of material modifications. Our data suggest that RIE modified polymer filters can isolate model cell lines while retaining ell viability, and that themore » RIE filter modification allows T24 monolayering cells to proliferate as a structured cluster. Copyright 2016 The Authors. Published by Elsevier B.V. All rights reserved.« less

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1392437
DOE Contract Number:  
AC02-06CH11357
Resource Type:
Journal Article
Journal Name:
Materials Science and Engineering. C, Biomimetic Materials, Sensors and Systems
Additional Journal Information:
Journal Volume: 66; Journal ID: ISSN 0928-4931
Publisher:
Elsevier
Country of Publication:
United States
Language:
English

Citation Formats

Makarova, Olga V., Adams, Daniel L., Divan, Ralu, Rosenmann, Daniel, Zhu, Peixuan, Li, Shuhong, Amstutz, Platte, and Tang, Cha-Mei. Polymer microfilters with nanostructured surfaces for the culture of circulating cancer cells. United States: N. p., 2016. Web. doi:10.1016/j.msec.2016.04.075.
Makarova, Olga V., Adams, Daniel L., Divan, Ralu, Rosenmann, Daniel, Zhu, Peixuan, Li, Shuhong, Amstutz, Platte, & Tang, Cha-Mei. Polymer microfilters with nanostructured surfaces for the culture of circulating cancer cells. United States. https://doi.org/10.1016/j.msec.2016.04.075
Makarova, Olga V., Adams, Daniel L., Divan, Ralu, Rosenmann, Daniel, Zhu, Peixuan, Li, Shuhong, Amstutz, Platte, and Tang, Cha-Mei. 2016. "Polymer microfilters with nanostructured surfaces for the culture of circulating cancer cells". United States. https://doi.org/10.1016/j.msec.2016.04.075.
@article{osti_1392437,
title = {Polymer microfilters with nanostructured surfaces for the culture of circulating cancer cells},
author = {Makarova, Olga V. and Adams, Daniel L. and Divan, Ralu and Rosenmann, Daniel and Zhu, Peixuan and Li, Shuhong and Amstutz, Platte and Tang, Cha-Mei},
abstractNote = {There is a critical need to improve the accuracy of drug screening and testing through the development of in vitro culture systems that more effectively mimic the in vivo environment. Surface topographical features on the nanoscale level, in short nanotopography, effect the cell growth patterns, and hence affect cell function in culture. We report the preliminary results on the fabrication, and subsequent cellular growth, of nanoscale surface topography on polymer microfilters using cell lines as a precursor to circulating tumor cells (CTCs). To create various nanoscale features on the microfilter surface, we used reactive ion etching (RIE) with and without an etching mask. An anodized aluminum oxide (AAO) membrane fabricated directly on the polymer surface served as an etching mask. Polymer filters with a variety of modified surfaces were used to compare the effects on the culture of cancer cell lines in blank culture wells, with untreated microfilters or with RIE-treated microfilters. We then report the differences of cell shape, phenotype and growth patterns of bladder and glioblastoma cancer cell lines after isolation on the various types of material modifications. Our data suggest that RIE modified polymer filters can isolate model cell lines while retaining ell viability, and that the RIE filter modification allows T24 monolayering cells to proliferate as a structured cluster. Copyright 2016 The Authors. Published by Elsevier B.V. All rights reserved.},
doi = {10.1016/j.msec.2016.04.075},
url = {https://www.osti.gov/biblio/1392437}, journal = {Materials Science and Engineering. C, Biomimetic Materials, Sensors and Systems},
issn = {0928-4931},
number = ,
volume = 66,
place = {United States},
year = {Thu Sep 01 00:00:00 EDT 2016},
month = {Thu Sep 01 00:00:00 EDT 2016}
}